Anti-βIII Tubulin antibody ValidAbTM
Product overview
Name | Anti-βIII Tubulin antibody ValidAbTM |
Description | Antibody to ß3 Tubulin - cytoskeletal protein used as a neuronal marker. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
Host | Mouse |
Clonality | Monoclonal |
Target | Beta III tubulin |
Validation data
Product information
Immunogen | Amino acids 441-448 of human beta III tubulin coupled to maleimide-activated keyhole limpet hemocyanin |
Epitope | ESESQGPK (Amino acids 441-448 of beta III tubulin) |
Clone number | TU-20 |
Isotype | IgG1 |
Purification | Protein A affinity chromatography |
Concentration | 1mg/ml |
Formulation | Lyophilised. When reconstituted contains PBS with 15mM sodium azide and 1% recombinant BSA |
Predicted species reactivity | Human, Mouse, Rat, Pig, Dog |
Tested species reactivity | Mouse, Rat |
Tested applications
Applications | WB, IHC(IF) |
Western blot optimal concentration | 1µg/ml (1:1000) as measured in rat brain cytosol |
IHC(IF) optimal concentration | 1µg/ml (1:1000) as measured in free-floating fixed hippocampal sections |
Positive control | ß3-tubulin is widely expressed in neural tissues. It is also well expressed in SH-SY5Y, Hep G2, A549 and SCLC-21H cell lines. |
Negative control | Non-neural tissues, except for tissue from the testes. Poorly expressed in many cell lines such as JURKAT, HeLa and HEK293. |
Open data link | Please follow this link to OSF |
Target information
Other names | TUBB3, Tubulin beta-4 chain, Tubulin beta-III |
UniProt ID | Q13509 |
Structure image | |
Gene name | TUBB3 |
NCBI full gene name | tubulin beta 3 |
Entrez gene ID | 10381 |
Amino acids | 450 (50.4kDa) |
Isoforms | Beta III tubulin has two isoforms. Isoform 1: canonical; Isoform 2: missing amino acids 1-72 |
Expression | Beta III tubulin is expressed almost exclusively within neurones present in the central nervous system and peripheral nervous system. Expression has also been found within the sertoli cells of the testes. |
Subcellular expression | Beta III tubulin is a key cytoskeletal component therefore is widely expressed as bundles of Beta III tubulin positive fibres. |
Processing | Following translation no processing is required for Beta III tubulin to reach its active conformation. |
Post translational modifications | Beta III tubulin is subject to three postranslational modifications: phosphorylation by CDK1 at Ser172, Polyglutamylation at Glu438 and phosphorylation at Ser 444 (note: this is within the epitope of HB6639) |
Homology (compared to human) | Mouse and human proteins are identical while rat beta III tubulin shows a single change (E440D) |
Similar proteins | Beta III tubulin shows similarity in a BLAST search to other beta tubulin family members (e.g. Tubulin beta IV 100%, tubulin beta VI 96%, tubulin beta IIA 95%, tubulin beta IIB 95%) alongside alpha tubulin (96% similarity) and epididymis sperm binding protein (95%) |
Epitope homology (between species) | The epitope sequence is conserved between humans, mice and rats within beta III tubulin |
Epitope homology (other proteins) | Proteins containing the sequence of the epitope of HB6639 include:
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Storage & Handling
Storage instructions | -20°C then use reconstitution advice |
Reconstitution advice | We recommend reconstituting with either:
Take care when opening as the precipitate is extremely light and can easily be lost if disturbed. When reconstituting make sure that the antibody is thoroughly dissolved by pipetting up and down before giving the antibody a brief spin at <10,000g to make sure that all material is recovered and at the bottom of the tube. For more information please see our detailed guide on storing and using your antibody |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
References for Anti-βIII Tubulin antibody ValidAbTM
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Human TUBB3 mutations perturb microtubule dynamics, kinesin interactions, and axon guidance
Tischfield M et al (2011) Cell 140(1) : 74-87 -
Mutations in the neuronal β-tubulin subunit TUBB3 result in malformation of cortical development and neuronal migration defects
Poirier K et al (2010) Human Molecular Genetics 19(22) : 4462–4473 -
Class III β-tubulin expression and in vitro resistance to microtubule targeting agents
Stengel C et al (2010 ) British Journal of Cancer 102(2) : 316-24 -
Proteomic characterization of cytoskeletal and mitochondrial class III beta-tubulin
Cicchillitti L et al (2008 ) Mol Cancer Ther 7(7) : 2070-9