MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous)

(HB9417)
Technical documents: Datasheet

Product overview

Name MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous)
Biological description

Overview

MightyMountTM Antifade Fluorescence Mounting Medium with phalloidin-TRITC (aqueous) is an ideal formulation for prevention of photobleaching of fluorescent proteins and dyes during fluorescent imaging. It is easy to use with an ideal refractive index and provides effective prevention of photobleaching. This formulation contains Phalloidin-TRITC which is a widely used red-orange fluorescent cytoskeleton stain which binds and labels F-actin.

Applications: IHC(IF), ICC, Cellular imaging, Super-resolution microscopy
Mounting: Aqueous (non-setting)
Antifade: Yes
Counterstain: Phalloidin-TRITC
Refractive index: 1.45


Other Mounting Media Products

We supply a full range of mounting media for a range of experimental needs:

Hardset:

Aqueous:

Description

Antifade aqueous fluorescence mounting medium with Phalloidin-TRITC for use in IHC(IF) and ICC.

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Images

Figure 1. β-tubulin staining in HEK293T cells. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous).

4% PFA fixed HEK293T cells were stained for β-tubulin (HB6491) and mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous). For a full protocol and more information on ICC please see our ICC protocol.

Figure 2. Fixed HEK293T cells mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous).

4% PFA fixed HEK293T cells were mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous). For a full protocol and more information on ICC please see our ICC protocol.

Figure 3. β-tubulin staining in HEK293T cells. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous).

4% PFA fixed HEK293T cells were stained for β-tubulin (HB6491) and mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous). For a full protocol and more information on ICC please see our ICC protocol.

Figure 4. Antifade performance of MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Hello Bio MightyMountTM Antifade Fluorescence Mounting Medium (aqeous) shows strong antifade characteristics against a range of fluorophores and compares favorably against VECTASHIELD® and Fluoromount-G™. Method: HEK293T cells were cultured on coverslips then stained with DAPI (HB0747) and with a mouse anti-β-tubulin antibody (HB6491) with Alexa Fluor 488 or DyLight 550 conjugated secondary antibodies. Following mounting with either MightyMountTM Antifade Fluorescence Mounting Medium (aqueous), VECTASHIELD® or Fluoromount-G™, slides were imaged on a confocal microscope. Imaging was carried out as a timeseries at 100% laser power with the normalized intensity being calculated compared to the first exposure.

Figure 5. Fixed HEK293T cells mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous).

4% PFA fixed HEK293T cells were mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous). For a full protocol and more information on ICC please see our ICC protocol.

Figure 6. β-tubulin staining in HEK293T cells. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous).

4% PFA fixed HEK293T cells were stained for β-tubulin (HB6491) and mounted using MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous). For a full protocol and more information on ICC please see our ICC protocol.

Optical Data

Emission color Red
Max excitation wavelength 540nm
Max emission wavelength 565nm
Cell permeable Yes

Biological Data

Application notes

Protocol for use of mounting media

IHC(IF)

  1. Mount sections onto subbed or charged microscope slides and air dry (in the dark) until sections are moist but all excess liquid has evaporated
  2. Add a few drops of mounting media around the sections (around 50µl but this will depend on the number and thickness of sections) and slowly lower the coverslip from one end of the slide to the other being careful to avoid creating any bubbles.
  3. Use clear nail varnish to seal the edges of the slide to avoid movement during imaging and stop evaporation.

For more information on IHC(IF) including tips on how to mount sections, please see our IHC(IF) protocol

 

ICC

  1. Add a drop of mounting medium (Around 5µl for a 10mm and 15µl for a 22mm coverslip) to a standard microscope slide.
  2. Briefly rinse the coverslip in dH2O before placing face down into the drop of mounting medium being careful not to introduce bubbles.
  3. Use clear nail varnish to seal the edges of the coverslip to avoid movement during imaging and stop evaporation.

For more information on ICC please see our ICC protocol

Solubility & Handling

Storage instructions

+4°C or -20°C long-term. Protect from light.

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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