MightyMount^TM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (hardset)

Protocol for use of mounting media

Once mounted, store slides at 4°C in the dark for optimal preservation of fluorescence.

IHC(IF)

1. Mount sections onto subbed or charged microscope slides and air dry (in the dark) until sections are moist but all excess liquid has evaporated
2. Add a few drops of mounting media around the sections (around 50µl but this will depend on the number and thickness of sections) and slowly lower the coverslip from one end of the slide to the other being careful to avoid creating any bubbles.
3. Wrap slides in foil to prevent light exposure then allow the media to cure at 4°C overnight before imaging. If more rapid imaging is needed it is possible to accelerate the curing process by incubating slides at either room temperature or 37°C for ≈1 hour.

For more information on IHC(IF) including tips on how to mount sections, please see our IHC(IF) protocol

ICC

1. Add a drop of mounting medium (Around 5µl for a 10mm and 15µl for a 22mm coverslip) to a standard microscope slide.
2. Briefly rinse the coverslip in dH₂O before placing face down into the drop of mounting medium being careful not to introduce bubbles.
3. Wrap slides in foil to prevent light exposure then allow the media to cure at 4°C overnight before imaging. If more rapid imaging is needed it is possible to accelerate the curing process by incubating slides at either room temperature or 37°C for ≈1 hour.

For more information on ICC please see our ICC protocol

+4°C or -20°C long-term. Protect from light.