|Description||Blue fluorescent DNA stain. Nuclear counterstain.|
|Biological description|| |
DAPI is a blue fluorescent DNA stain which is cell permeant at high concentrations.
DAPI binds strongly to A-T rich regions in DNA to form a fluorescent complex. It preferentially stains ds-DNA and has a high quantum yield (φf=0.92) when bound to DNA.
Uses and applications
DAPI is commonly used as a nuclear and chromosome counterstain.
It is preferentially used to stain dead cells. DAPI is less effective as a live cell stain as it is unable to efficiently pass through the membrane in live cells. Therefore, higher concentrations may need to be used.
Cells must be permeabilized and/or fixed for DAPI to enter the cell and bind to DNA.
Due to DAPI’s blue emission, there is very little fluorescent overlap between yellow-fluorescent, green-florescent molecules (e,g, fluorescein and GFP) or red-fluorescent stains (e.g. Texas red). It is therefore convenient for multiplexing assays.
DAPI has a great variety of applications but is often used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis and in HCA (high-content analysis).
Solubility & Handling
|Solubility overview||Soluble in water (10mg/ml, gentle warming), and in methanol|
|Important||This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.|
|Chemical name||4',6-Diamidino-2-phenylindole dihydrochloride|
|Excitation||340 / 360nM (for ds-DNA)|
|Emission||488 / 460nM (for ds-DNA)|
References for DAPI
DAPI: a DNA-specific fluorescent probe.Kapuscinski J (1995) Biotech Histochem 70(5) : 220-33.
Labeling nuclear DNA using DAPI.Chazotte B (2011) Cold Spring Harb Protoc 2011(1) : pdb.prot5556.
New insights into the in situ microscopic visualization and quantification of inorganic polyphosphate stores by 4',6-diamidino-2-phenylindole (DAPI)-staining.Gomes FM et al (2013) Eur J Histochem 57(4) : e34.
DAPI as a useful stain for nuclear quantitation.Tarnowski et al (1991) Biotech Histochem 66(6) : 297-302
Labeling nuclear DNA using DAPI.Chazotte et al (2011) Cold Spring Harb Protoc 2011(1) : 5556