Figure. 1: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183 nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure. 2: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure. 3: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1µg/ml). For protocol see #Protocol 1 in application notes below.
Figure. 1: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183 nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure. 2: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.
Figure. 3: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.
Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1µg/ml). For protocol see #Protocol 1 in application notes below.
Biological Data
Application notes
For our 300 tests pack, to make your stock solution, you should dissolve the contents of the vial in 1.5 mL of methanol or DMSO.
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#Protocol 1: Rhodamine Phalloidin TRITC labelling of primary cultured neurones.
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Primary neurones were isolated and cultured from P2 rats and grown for three weeks before being fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
Coverslips were incubated for 1 hour with Rhodamine Phalloidin TRITC (183nM, 1:40 dilution of staining solution)
Coverslips were then submerged in 1µg/ml DAPI diluted in PBS for 1 minute.
Coverslips were mounted and imaged with a fluorescent microscope.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO (NB: may appear colourless in very dry solvent)
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
![Rhodamine phalloidin-TRITC [1926163-50-9] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb8621.png "Rhodamine phalloidin-TRITC [1926163-50-9]")
Understanding purity and quality - a guide for life scientists
