Rhodamine phalloidin-TRITC

(HB8621)
Technical documents: SDS CoA Datasheet
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Product overview

Name Rhodamine phalloidin-TRITC
Biological description

Red-orange fluorescent cytoskeleton stain which binds and labels F-actin.

Has a wide range of applications and can be used in tissue sections, cell cultures and cell-free experiments samples.

Purity >95%
Description Red-orange fluorescent cytoskeleton stain.
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Images

Figure. 1: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.

Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183 nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.

Figure. 2: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.

Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.

Figure. 3: Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture.

Rhodamine Phalloidin TRITC and DAPI co-staining in neuronal cell culture. Rhodamine Phalloidin TRITC shows high affinity for F-actin therefore is used to label actin filaments in immunofluorescence experiments. Rhodamine Phalloidin TRITC from Hello Bio labels actin filaments (red) at 183nM when counterstained with DAPI (blue — HB0747, 1µg/ml). For protocol see #Protocol 1 in application notes below.

Biological Data

Application notes

For our 300 tests pack, to make your stock solution, you should dissolve the contents of the vial in 1.5 mL of methanol or DMSO.

 

#Protocol 1: Rhodamine Phalloidin TRITC labelling of primary cultured neurones.

 

  • Primary neurones were isolated and cultured from P2 rats and grown for three weeks before being fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
  • Coverslips were incubated for 1 hour with Rhodamine Phalloidin TRITC (183nM, 1:40 dilution of staining solution)
  • Coverslips were then submerged in 1µg/ml DAPI diluted in PBS for 1 minute.
  • Coverslips were mounted and imaged with a fluorescent microscope.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO (NB: may appear colourless in very dry solvent)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Chemical name 7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin
Molecular Weight 1231.4
Chemical structure Rhodamine phalloidin-TRITC [1926163-50-9] Chemical Structure
Molecular Formula C60H70N12O13S2
CAS Number 1926163-50-9
PubChem identifier 137247539
SMILES C[C@H]1C(=O)N[C@H]2CC3=C(NC4=CC=CC=C34)SC[C@@H](C(=O)N5C[C@H](C[C@H]5C(=O)N1)O)NC(=O)[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC2=O)C[C@](C)(CNC(=S)NC6=CC(=C(C=C6)C(=O)[O-])C7=C8C=CC(=[N+](C)C)C=C8OC9=C7C=CC(=C9)N(C)C)O)C)[C@H](C)O
InChiKey NRDVPFYYTLFSBJ-IZZNSDNCSA-N
MDL number MFCD30748671
Excitation ~540
Emission 565 nm

References for Rhodamine phalloidin-TRITC

References are publications that support the biological activity of the product
  • Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

    Chazotte B (2010) Cold Spring Harb Protoc 2010(5) : pdb.prot4947
  • Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin

    Kudryashov DS et al (2004) Biophys J 87(2) : 1136-45
  • Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin

    Kudryashov DS et al (2004) Biophys J 87(2) : 1136-45
  • Distribution and orientation of rhodamine-phalloidin bound to thin filaments in skeletal and cardiac myofibrils

    Zhukarev V et al (1997) Cell Motil Cytoskeleton 37(4) : 363-77

4 Item(s)

Publications
These publications cite the use of Rhodamine phalloidin-TRITC purchased from Hello Bio:
  • Unconjugated p-cresol activates macrophage macropinocytosis leading to increased LDL uptake

    Chaves LD et al (2021) JCI Insight 6(11)
    PubMedID: 33914709

1 Item