Choosing the Right Neuronal & Glial Markers

A human brain contains around 86 billion neurones and 85 billion non-neural cells. Within this vast array of cells is a remarkable degree of heterogeneity and specialisation which are determined by the differential expression of thousands of gene products. This differential protein expression between cell types is used to identify specific cell types using marker antibodies. At Hello Bio we provide a range of markers to identify different cell types found in the central nervous system.

General neuronal markers

Neurons are the central information processing unit in the nervous system and have remarkable heterogeneity dependent upon their location and function. Various neural markers are available that express in different cellular compartments and neuron subtypes. We have curated a collection of general use highly reliable antibodies that are excellent markers of neurons.

General neuronal markers:
MAP2
Microtubule stabilising protein only expressed in neurones.
HB9587 – Mouse monoclonal
NeuN
NeuN
Transcription factor expressed in the nuclei of mature neurones.
Note: Some neuronal subtypes such as Purkinje, stellate and Golgi cells do not express NeuN
HB6429 – Mouse monoclonal
β3-Tubulin
Cytoskeletal component that expresses early in the neuronal differentiation process.
HB6639 – Mouse monoclonal

 

Neurofilament L
Component of intermediate filaments that stabilise neuronal structure
HB6433 – Mouse monoclonal
Neurofilament M
Neurofilament M
Component of intermediate filaments that stabilise neuronal structure
HB7849 – Mouse monoclonal

 

Glial Markers: astrocyte markers

Astrocytes are a sub-type of glial cell whose principal role is to support the healthy function of neurones in the nervous system. Astrocytes perform this role through carrying out a range of activities including but not limited to supplying glucose and lactate to neurones, providing structural support, uptake and release of various neurotransmitters and regulating local ion concentration.

GFAP
GFAP
Component of intermediate filaments and only expressed in astroycytes.
HB8267 – Mouse monoclonal