FITC Phalloidin

(HB0814)
Technical documents: SDS CoA Datasheet
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Product overview

Name FITC Phalloidin
Alternative names Fluorescein Phalloidin, Phalloidin Fluorescein Isothiocyanate Labeled, Phalloidin (FITC), Fl-phalloidin
Biological description

Overview

Fluorescein Isothiocyanate (FITC) labeled Phalloidin binds and labels F-actin but not G-actin.

It is a green fluorescent stain which allows high-contrast discrimination of actin. Non-specific binding of phalloidin is negligible and it provides more intense labeling of F-actin than antibodies.

Uses and applications

FITC Phalloidin has a wide range of applications and can be used with formaldehyde-fixed and permeabilized tissue sections, cell cultures and cell-free experiments samples. It may also be used with de-paraffinized paraffin-embedded samples.

Purity >93%
Description Green fluorescent cytoskeleton stain. Binds and labels F-actin.
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Images

Figure 1. FITC Phalloidin and DAPI co-staining in a fibroblast observed in neuronal cell culture

FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when counterstained with DAPI (blue — HBO747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.

Figure 2. FITC Phalloidin, GFAP and DAPI co-staining in neuronal cell culture

FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HBO0747, 1 µg/ml). For protocol see #Protocol 1 in application notes below.

Figure 3. FITC Phalloidin, GFAP and DAPI co- staining in neuronal cell culture

FITC Phalloidin shows high affinity for actin therefore is used to label actin filaments in immunofluorescence experiments. FITC Phalloidin from Hello Bio labels actin filaments (green) at 183 nM when co-stained with an anti-GFAP antibody (red) and DAPI (blue — HB0747, µg/ml). For protocol see #Protocol 1 in application notes below.
FITC Phalloidin product vial image | Hello Bio

Biological Data

Application notes

For our 300 tests pack, to make your stock solution, you should dissolve the contents of the vial in 1.5 mL of methanol or DMSO.

 

#Protocol 1: FITC-Phalloidin labelling of primary cultured neurones.

 

  • Primary neurones were isolated and cultured from P2 rats and grown for three weeks before being fixed with 4% paraformaldehyde.
  • Coverslips containing neuronal cell cultures were labelled for GFAP following standard immunohistochemical approaches.
  • The secondary antibody was incubated for 1 hour with the addition of FITC Phalloidin (183nM, 1:40 dilution of staining solution)
  • Coverslips were then submerged in 1µg/ml DAPI diluted in PBS for 1 minute.
  • Coverslips were mounted and imaged with a fluorescent microscope.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO (NB: may appear colourless in very dry solvent)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >93%
Chemical name 7-[(4R)-N5-[[(3,6-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
Molecular Weight 1177.26
Chemical structure Phalloidin, Fluorescein Isothiocyanate Labeled | [] Chemical Structure
Molecular Formula C56H60N10O15S2
CAS Number 915026-99-2
PubChem identifier 347679539
SMILES S=C(NCC1CSC(C)(S1)CC1NC(=O)C2NC(=O)C(C)NC(=O)C3CC(O)CN3C(=O)C(CSc3[nH]c4ccccc4c3C2)NC(=O)C(NC(=O)C(C)NC1=O)C(C)O)Nc1ccc2c(c1)C(=O)OC12c2ccc(O)cc2Oc2cc(O)ccc12
InChiKey WUSBHBKXQMYBEH-ADESOLLTSA-N
MDL number MFCD00147902
Excitation 496
Emission 516 nm

References for FITC Phalloidin

References are publications that support the biological activity of the product
  • Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons

    Li H et al (2016) J Vis Exp 108 : e53697
  • Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

    Chazotte B (2010) Cold Spring Harb Protoc 2010(5) : pdb.prot4947
  • Cytoskeletal F-actin patterns quantitated with fluorescein isothiocyanate-phalloidin in normal and transformed cells

    Verderame M et al (1980) Proc Natl Acad Sci U S A 77(11) : 6624-8

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