MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous)

Name: MightyMount TM Antifade Fluorescence Mounting Medium (aqueous)
Brand: hello bio
SKU: HB9854
Price: 114 USD
Availability: InStock

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Product overview

Name

MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous)

Biological description

Overview

MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) is an ideal formulation for prevention of photobleaching of fluorescent proteins and dyes during fluorescent imaging. It is easy to use with an ideal refractive index and provides effective prevention of photobleaching.

Applications: IHC(IF), ICC, Cellular imaging, Super-resolution microscopy
Mounting: Aqueous (non-setting)
Antifade: Yes
Counterstain: None
Refractive index: 1.45

Other Mounting Media Products

We supply a full range of mounting media for a range of experimental needs:

Hardset:

Aqueous:

Description

Antifade aqueous fluorescence mounting medium for use in IHC(IF) and ICC.

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Images

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

4% PFA fixed 40µm horizontal rat brain sections were stained for GFAP (HB8267) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.

Figure 4. Antifade performance of MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Hello Bio MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) shows strong antifade characteristics against a range of fluorophores and compares favorably against VECTASHIELD® and Fluoromount-G™. Method: HEK293T cells were cultured on coverslips then stained with DAPI (HB0747) and with a mouse anti-β-tubulin antibody (HB6491) with Alexa Fluor 488 or DyLight 550 conjugated secondary antibodies. Following mounting with either MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous), VECTASHIELD® or Fluoromount-G™, slides were imaged on a confocal microscope. Imaging was carried out as a timeseries at 100% laser power with the normalized intensity being calculated compared to the first exposure.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Glyoxal fixed (9% glyoxal, 8% acetic acid, pH4) 40µm horizontal rat brain sections were stained for GluN1 (HB7535) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

4% PFA fixed 40µm horizontal rat brain sections were stained for Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium (aqueous).

Biological Data

Application notes

Protocol for use of mounting media

IHC(IF)

Mount sections onto subbed or charged microscope slides and air dry (in the dark) until sections are moist but all excess liquid has evaporated
Add a few drops of mounting media around the sections (around 50µl but this will depend on the number and thickness of sections) and slowly lower the coverslip from one end of the slide to the other being careful to avoid creating any bubbles.
Use clear nail varnish to seal the edges of the slide to avoid movement during imaging and stop evaporation.

For more information on IHC(IF) including tips on how to mount sections, please see our IHC(IF) protocol

ICC

Add a drop of mounting medium (Around 5µl for a 10mm and 15µl for a 22mm coverslip) to a standard microscope slide.
Briefly rinse the coverslip in dH₂O before placing face down into the drop of mounting medium being careful not to introduce bubbles.
Use clear nail varnish to seal the edges of the coverslip to avoid movement during imaging and stop evaporation.

For more information on ICC please see our ICC protocol

Solubility & Handling

Storage instructions

+4°C or -20°C long-term. Protect from light.

Important

This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Antifade aqueous fluorescence mounting medium for use in IHC(IF) and ICC.

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Order: HB9854
Pack	Price	Qty
10mL	$114

MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous)

Product overview

Images

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Biological Data

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Calculators

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New Products in this Area

MightyMountTM Antifade Fluorescence Mounting Medium (aqueous)

Product overview

Images

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).

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MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous)

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 4. Antifade performance of MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.

Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).

Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMount^TM Antifade Fluorescence Mounting Medium (aqueous).