Anti-HSP60 antibody ValidAbTM
Product overview
Name | Anti-HSP60 antibody ValidAbTM |
Description | Antibody to HSP60 - loading control and mitochondrial marker for immunohistochemistry and immunocytochemistry. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
Host | Mouse |
Clonality | Monoclonal |
Target | HSP60 |
Validation data
Product information
Immunogen | This antibody was a spontaneous auto-antibody therefore does not have an immunogen |
Epitope | Amino acids 390-409 of human HSP60 (LNERLAKLSDGVAVLKVGGT) |
Clone number | 1C7 |
Isotype | IgG1 |
Purification | Protein G affinity chromatography |
Concentration | 1mg/ml |
Formulation | 50% PBS, 50% glycerol + 5mM sodium azide |
Predicted species reactivity | Mouse, Rat, Human, Cat, Chicken, Cow, Dog, Fish, Horse, Monkey, Pig, Rabbit, Turkey |
Tested species reactivity | Mouse, Rat, Human |
Tested applications
Applications | WB, IHC(IF) |
Western blot optimal concentration | 100ng/ml (1:10,000 dilution) as measured in rat brain P2 fraction |
IHC(IF) optimal concentration | 250ng/ml (1:4,000 dilution) as measured in rat hippocampal sections |
Positive control | HSP60 is ubiquitously expressed in the mitochondria of nearly all mammalian cells and tissues. It is also widely expressed in common cell lines. |
Negative control | HSP60 is a mitochondrial enzyme so complete subcellular fractionation should be sufficient to provide a negative control. Due to its high expression, care should be taken to ensure that fractionation is complete without any mitochondrial contamination. |
Open data link | Please follow this link to OSF |
Target information
Other names | 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, GroEL |
UniProt ID | P10809 |
Structure image | |
Gene name | HSPD1 |
NCBI full gene name | heat shock protein family D (Hsp60) member 1 |
Entrez gene ID | 3329 |
Amino acids | 573 (61.1kDa) |
Isoforms | HSP60 has two isoforms:- Isoform 1 (canonical), 573aa, 61.1kDa; isoform 2, 158aa, 17.1kDa, substitution between residues 144 and 158 (VMLAVDAVIAELKKQ → RNVCCHHSVLNFSV) and deletion of residues 159-573. |
Expression | Expressed ubiquitously in all tissues |
Subcellular expression | Primarily expressed in the mitochondria but recent evidence suggests it is also expressed in the cytosol, cell surface and extracelular space. |
Processing | Amino acids 1-26 are a mitcochondrial localisation tag which are removed from the completed protein. |
Post translational modifications | HSP60 is subject to numerous post-translational modifications including acetylation, succinylation and phosphorylation |
Homology (compared to human) | Mouse and rat show 97.56% similarity to human HSP60 in a BLAST search |
Similar proteins | Nearest similarity protein is T-complex protein 1 subunit epsilon at 26% identity. |
Epitope homology (between species) | The epitope is 100% conserved between mouse, rat and human HSP60 sequences. |
Epitope homology (other proteins) | No proteins have sigificant homology to the epitope. The closest matches are: cTAGE-2 human 66.7% identity, Olfactory receptor 6C70 human 71.4% identity |
Storage & Handling
Storage instructions | -20°C |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
References for Anti-HSP60 antibody ValidAbTM
-
Hsp60 expression, new locations, functions and perspectives for cancer diagnosis and therapy
Cappello et al (2008) Cancer Biol Ther 7(6) : 801-809 -
Heat-shock proteins as activators of the innate immune system
Wallin R et al (2002) Trends in Immunology 23(3) : 130-135 -
The Hsp70 and Hsp60 chaperone machines
Bukau B and Horwich AL (1998) Cell 92(3) : 351-366 -
Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis
Ostermann J et al (1989) Nature 341(6238) : 125-130