Goat Anti-Mouse IgG H&L (Janelia Fluor® 646) preadsorbed ValidAb™
(HB7393)
Product overview
| Name | Goat Anti-Mouse IgG H&L (Janelia Fluor® 646) preadsorbed ValidAb™ |
| Host | Goat |
| Clonality | Polyclonal |
| Target | Mouse IgG H&L |
| Conjugate | Janelia Fluor® 646 |
| Description | Goat Anti-Mouse IgG H&L Janelia Fluor® 646 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
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Validation data
Figure 1. Vimentin expression in HEK293T cells visualised using HB6497 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
HEK293T cells stained with for Vimentin (HB6497) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed as the secondary antibody. Method: HEK293 cells were cultured following established protocols (Lee et al., 2019. PLoS ONE, 14(5):e0213116) before being fixed with 4% PFA. Cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6497 (mouse monoclonal anti-vimentin) was incubated overnight (4°C) at a 1:2,000 dilution (0.5µg/ml) followed by a one hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody (1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured in Lightning deconvolution mode using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DMi8 inverted epifluorescence microscope with a 63x objective, 405nm (9.95% power, HyD: 10% gain) and 633nm (1.5% power, HyD: 10% gain) excitation lasers. Images were captured as a z-stack (0.3µm spacing) before being flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 2. Purkinje neurons stained for βIII tubulin in rat cerebellum using HB6639 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for β3-tubulin (HB6639) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB6639 (1:1,000 dilution, 1µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured as a z-stack (0.3µm spacing) using a Leica DMI6000B fluorescence microscope with a 40x objective using DAP (5ms exposure) and Y5 (100ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 3. Vimentin expression in HEK293T cells visualised using HB6497 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
HEK293T cells stained with for Vimentin (HB6497) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed as the secondary antibody. Method: HEK293 cells were cultured following established protocols (Lee et al., 2019. PLoS ONE, 14(5):e0213116) before being fixed with 4% PFA. Cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6497 (mouse monoclonal anti-vimentin) was incubated overnight (4°C) at a 1:2,000 dilution (0.5µg/ml) followed by a one hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody (1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured in Lightning deconvolution mode using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DMi8 inverted epifluorescence microscope with a 63x objective, 405nm (9.95% power, HyD: 10% gain) and 633nm (2.8% power, HyD: 10% gain) excitation lasers. Images were captured as a z-stack (0.3µm spacing) before being flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 4. Janelia Fluor® 646 conjugated secondary antibodies show superior antifade performance with equivalent brightness compared to those conjugated with DyLight 650
Janelia Fluor® 646 conjugated secondary antibodies show greater resistance to photobleaching alongside equivalent brightness than DyLight 650 conjugated secondaries in a HEK293T based cell assay. Method: HEK293T cells were cultured on 10mm coverslips and stained with an anti-MFF antibody (1:1,000 dilution) and either a goat anti-rabbit DyLight 650 or goat anti-rabbit Janelia Fluor® 646 conjugated secondary antibody. For more information please see our ICC protocol. Imaging was conducted with a Leica SPE confocal laser scanning microscope where cells were repeatedly imaged with the 635nm laser at 100% power over 84 seconds of continuous exposure to induce photobleaching. Average intensity values were calculated from images then data was normalised to the first exposure frame to generate normalised intensity. Statistics: decay curve: F-test, F1,896=1315, p<0.0001, final intensity: two tailed t-test, t8=10.74, p<0.0001, initial brightness: two tailed t-test, t8=0.13, p=0.90
Figure 5. Rat cerebellum stained for MBP (HB8014) and Calretinin (HB6494) using HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646) and HB9317 Goat Anti-Rabbit H&L (AF488).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) and calretinin (HB6494) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml) and HB6494 (1:2,000 dilution). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure), L5 (200ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 6. Purkinje neurons stained for βIII tubulin in rat cerebellum using HB6639 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for β3-tubulin (HB6639) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB6639 (1:1,000 dilution, 1µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured as a z-stack (0.3µm spacing) using a Leica DMI6000B fluorescence microscope with a 40x objective using DAP (5ms exposure) and Y5 (100ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 7. Cerebellum stained for Myelin basic protein (MBP) using HB8014 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 8. Rat cerebellum stained for MBP (HB8014) and Calretinin (HB6494) using HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646) and HB9317 Goat Anti-Rabbit H&L (AF488).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) and calretinin (HB6494) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml) and HB6494 (1:2,000 dilution). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure), L5 (200ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 1. Vimentin expression in HEK293T cells visualised using HB6497 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
HEK293T cells stained with for Vimentin (HB6497) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed as the secondary antibody. Method: HEK293 cells were cultured following established protocols (Lee et al., 2019. PLoS ONE, 14(5):e0213116) before being fixed with 4% PFA. Cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6497 (mouse monoclonal anti-vimentin) was incubated overnight (4°C) at a 1:2,000 dilution (0.5µg/ml) followed by a one hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody (1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured in Lightning deconvolution mode using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DMi8 inverted epifluorescence microscope with a 63x objective, 405nm (9.95% power, HyD: 10% gain) and 633nm (1.5% power, HyD: 10% gain) excitation lasers. Images were captured as a z-stack (0.3µm spacing) before being flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 2. Purkinje neurons stained for βIII tubulin in rat cerebellum using HB6639 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for β3-tubulin (HB6639) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB6639 (1:1,000 dilution, 1µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured as a z-stack (0.3µm spacing) using a Leica DMI6000B fluorescence microscope with a 40x objective using DAP (5ms exposure) and Y5 (100ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 3. Vimentin expression in HEK293T cells visualised using HB6497 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
HEK293T cells stained with for Vimentin (HB6497) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed as the secondary antibody. Method: HEK293 cells were cultured following established protocols (Lee et al., 2019. PLoS ONE, 14(5):e0213116) before being fixed with 4% PFA. Cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6497 (mouse monoclonal anti-vimentin) was incubated overnight (4°C) at a 1:2,000 dilution (0.5µg/ml) followed by a one hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody (1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured in Lightning deconvolution mode using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DMi8 inverted epifluorescence microscope with a 63x objective, 405nm (9.95% power, HyD: 10% gain) and 633nm (2.8% power, HyD: 10% gain) excitation lasers. Images were captured as a z-stack (0.3µm spacing) before being flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 4. Janelia Fluor® 646 conjugated secondary antibodies show superior antifade performance with equivalent brightness compared to those conjugated with DyLight 650
Janelia Fluor® 646 conjugated secondary antibodies show greater resistance to photobleaching alongside equivalent brightness than DyLight 650 conjugated secondaries in a HEK293T based cell assay. Method: HEK293T cells were cultured on 10mm coverslips and stained with an anti-MFF antibody (1:1,000 dilution) and either a goat anti-rabbit DyLight 650 or goat anti-rabbit Janelia Fluor® 646 conjugated secondary antibody. For more information please see our ICC protocol. Imaging was conducted with a Leica SPE confocal laser scanning microscope where cells were repeatedly imaged with the 635nm laser at 100% power over 84 seconds of continuous exposure to induce photobleaching. Average intensity values were calculated from images then data was normalised to the first exposure frame to generate normalised intensity. Statistics: decay curve: F-test, F1,896=1315, p<0.0001, final intensity: two tailed t-test, t8=10.74, p<0.0001, initial brightness: two tailed t-test, t8=0.13, p=0.90
Figure 5. Rat cerebellum stained for MBP (HB8014) and Calretinin (HB6494) using HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646) and HB9317 Goat Anti-Rabbit H&L (AF488).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) and calretinin (HB6494) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml) and HB6494 (1:2,000 dilution). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure), L5 (200ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 6. Purkinje neurons stained for βIII tubulin in rat cerebellum using HB6639 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for β3-tubulin (HB6639) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB6639 (1:1,000 dilution, 1µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured as a z-stack (0.3µm spacing) using a Leica DMI6000B fluorescence microscope with a 40x objective using DAP (5ms exposure) and Y5 (100ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 7. Cerebellum stained for Myelin basic protein (MBP) using HB8014 and HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 8. Rat cerebellum stained for MBP (HB8014) and Calretinin (HB6494) using HB7393 Goat Anti-Mouse H&L (Janelia Fluor® 646) and HB9317 Goat Anti-Rabbit H&L (AF488).
Rat cerebellum stained for myelin basic protein (MBP, HB8014) and calretinin (HB6494) using HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies. Method: Rat brains were dissected and fixed overnight in 4% PFA before then being incubated in 30% sucrose (in PBS) until sunk (approx. 48hrs). A freezing microtome was used to cut 40µm horizontal slices before sections were incubated in 1% NaBH4 for 15 minutes. Sections were blocked in 0.05M glycine, 2% BSA and 3% goat serum before incubation overnight in HB8014 (1:2,000 dilution, 0.5µg/ml) and HB6494 (1:2,000 dilution). This was followed by a two-hour incubation with HB7393 goat anti-mouse IgG H&L (Janelia Fluor® 646) preadsorbed and HB9317 goat anti-rabbit IgG H&L (AF488) secondary antibodies at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our IHC(IF) protocol. The image was captured using a Leica DMI6000B fluorescence microscope with a 20x objective using DAP (40ms exposure), L5 (200ms exposure) and Y5 (220ms exposure) filters. Following capture, deconvolution was carried out using Huygens Professional before the stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Product information
| Immunogen | Purified mouse IgG |
| Purification | Immunogen affinity chromatography. Pre-adsorbed with bovine, horse, human, pig and rabbit serum proteins |
| Concentration | 1mg/ml |
| Formulation | 20% glycerol in PBS with 0.05% sodium azide and 1% recombinant albumin |
Tested applications
| Applications | ELISA, FACS and flow cytometry, ICC, IHC(IF) |
| IHC(IF) optimal concentration | 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml). |
| ICC optimal concentration | 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml). |
| Negative control | While this antibody has been cross-adsorbed to reduce non-specific binding it is still often worthwhile to conduct a control experiment where the primary antibody is omitted to give confidence that the staining pattern observed is specific. |
Optical Data
| Max excitation wavelength | 646 nm |
| Max emission wavelength | 664 nm |
| Emission color | Red |
| Closest laser lines | 640nm |
| Spectrally similar dyes | Alexa Fluor® 647, Atto 647, DyLight® 650, Cyanine 5 |
| Fluorescence spectra |  |
Storage & Handling
| Storage instructions | +4°C |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Technical guides
Paraffin Embedded IHC Protocol (IHC-P) (797.5 KB) Western Blot Protocol (1 MB) Immunocytochemistry Protocol (1.2 MB) Immunohistochemistry Protocol (1.6 MB) References for Goat Anti-Mouse IgG H&L (Janelia Fluor® 646) preadsorbed ValidAb™
References are publications that support the biological activity of the product
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Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.
Gonschior H et al (2022) Nature communications 13 : 4985 -
Single-molecule localization microscopy.
Lelek M et al (2021) Nature reviews. Methods primers 1 :
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