Product overview
Name | Propidium Iodide |
Description | Red-fluorescent cell viability stain |
Alternative names | PI |
Biological description | Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids.
Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
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Purity | >97% |
Images
Biological Data
Application notes | #Protocol 1: Propidium Iodide counterstaining of HEK293T cells
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Solubility & Handling
Storage instructions | +4°C |
Solubility overview | Soluble in water (5 mM) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Chemical name | 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide |
Molecular Weight | 668.39 |
Chemical structure | |
Molecular Formula | C27H34I2N4 |
CAS Number | 25535-16-4 |
PubChem identifier | 104981 |
SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
InChiKey | XJMOSONTPMZWPB-UHFFFAOYSA-M |
MDL number | MFCD00011921 |
Appearance | Purple solid |
Excitation | 535nM |
Emission | 617nM |
References for Propidium Iodide
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The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Banerjee A et al (2014) FEBS Open Bio 4 : 251-9. -
Analysis of apoptosis by propidium iodide staining and flow cytometry.
Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61. -
DNA staining for fluorescence and laser confocal microscopy.
Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.