Product overview
| Name | Propidium Iodide |
| Alternative names | PI |
| Biological description | Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids.
Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
|
| Purity | >97% |
| Description | Red-fluorescent cell viability stain |
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Images
Figure1. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium lodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti- vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 2. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 3. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 4. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Propidium Iodide product vial image | Hello Bio
Figure1. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium lodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti- vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 2. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 3. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Figure 4. Vimentin and Propidium lodide co-staining in HEK293T cells
Propidium lodide is a membrane-impermeable DNA binding dye used to label cell nuclei in immunofluorescence experiments. Propidium iodide from Hello Bio labels cell nuclei (red) at 1 µg/ml when co-stained with a anti-vimentin antibody (green). For protocol see #Protocol 1 in application notes below.
Propidium Iodide product vial image | Hello Bio
Biological Data
| Application notes | #Protocol 1: Propidium Iodide counterstaining of HEK293T cells
|
Solubility & Handling
| Storage instructions | +4°C |
| Solubility overview | Soluble in water (5 mM) |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Purity | >97% |
| Chemical name | 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide |
| Molecular Weight | 668.39 |
| Chemical structure | ![Propidium Iodide [25535-16-4] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0820.png "Propidium Iodide [25535-16-4]") |
| Molecular Formula | C27H34I2N4 |
| CAS Number | 25535-16-4 |
| PubChem identifier | 104981 |
| SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
| InChiKey | XJMOSONTPMZWPB-UHFFFAOYSA-M |
| MDL number | MFCD00011921 |
| Appearance | Purple solid |
| Excitation | 535nM |
| Emission | 617nM |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Propidium Iodide
References are publications that support the biological activity of the product
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The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Banerjee A et al (2014) FEBS Open Bio 4 : 251-9. -
Analysis of apoptosis by propidium iodide staining and flow cytometry.
Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61. -
DNA staining for fluorescence and laser confocal microscopy.
Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.
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