Red-fluorescent cell viability stain
|Biological description|| |
Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids.
Propidium iodide is membrane impermeant and is therefore frequently used to selectively identify dead cells and is commonly used in flow cytometry to evaluate cell viability.
Propidium iodide (PI) is often used in flow cytometry, fluorescent microscopy and confocal laser scanning microscopy applications.
Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Wavelength Maxima: Excitation ~535nm, Emission ~617nm
|Application notes|| |
#Protocol 1: Propidium Iodide counterstaining of HEK293T cells
- HEK293T cells were cultured on coverslips in 10% FBS in DMEM and fixed with 4% PFA. Immunocytochemistry was performed following our ICC protocol using an anti-vimentin monoclonal antibody at 1µg/ml.
- Prodidium iodide working solution was prepared consisting of 1µg/ml Propidium Iodide and 10µg/ml RNase A and incubated with cells for 30 minutes at room temperature.
- Following washing with PBS, coverslips were mounted and imaged using a confocal microscope using either a 532nm or 514nm laser for excitation
Solubility & Handling
|Storage instructions|| +4°C |
|Solubility overview|| Soluble in water (5 mM) |
|Important|| This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
|Chemical name|| 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide |
|Chemical structure|| |
|Molecular Formula|| C27H34I2N4 |
|PubChem identifier|| 104981 |
|SMILES|| CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
|InChiKey|| XJMOSONTPMZWPB-UHFFFAOYSA-M |
References for Propidium Iodide
References are publications that support the biological activity of the product
The DNA intercalators ethidium bromide and propidium iodide also bind to core histones.
Banerjee A et al (2014) FEBS Open Bio 4 : 251-9.
Analysis of apoptosis by propidium iodide staining and flow cytometry.
Riccardi C et al (2006) Nat Protoc 1(3) : 1458-61.
DNA staining for fluorescence and laser confocal microscopy.
Suzuki T et al (1997) J Histochem Cytochem 45(1) : 49-53.