Membrane permeable, red-shifted (Excitation 546nm, Emission 569nm), intracellular calcium (Ca2+) indicator (Kd = 310nM). Suitable for measurement of fast calcium dynamics in neurons and cardiomyocytes with excellent photostability and brightness compared to genetically encoded sensors. Reduces issues with tissue autofluorescence and background fluorescence due to the red-shifted fluorophore. Compatible with fluorescence microscopy using TRITC or Cy3 filters.
Figure 1. Optogenetically evoked calcium dynamics in human iPSC-derived cardiomyocytes
BAPTA-AM Janelia® Fluor 549 was used to successfully measure the calcium dynamics in cardiomyocytes in response to optogenetic stimulation. Method: Human iPSC-derived cardiomyocytes were virally transduced with channel rhodopsin and imaged 6 days following transfection. Cells were loaded with BAPTA-AM Janelia® Fluor 549 following the BAPTA-AM Janelia Fluor® 549 protocol. Recordings were made at 100Hz (Ex: 561nm, Em: 595/31nm) with a 10 second baseline period where cells exhibited spontaneous calcium transients before cells were stimulated optogenetically at 1.4Hz for a further 10 seconds. Recordings were made from two separate cells.
Figure 1. Optogenetically evoked calcium dynamics in human iPSC-derived cardiomyocytes
BAPTA-AM Janelia® Fluor 549 was used to successfully measure the calcium dynamics in cardiomyocytes in response to optogenetic stimulation. Method: Human iPSC-derived cardiomyocytes were virally transduced with channel rhodopsin and imaged 6 days following transfection. Cells were loaded with BAPTA-AM Janelia® Fluor 549 following the BAPTA-AM Janelia Fluor® 549 protocol. Recordings were made at 100Hz (Ex: 561nm, Em: 595/31nm) with a 10 second baseline period where cells exhibited spontaneous calcium transients before cells were stimulated optogenetically at 1.4Hz for a further 10 seconds. Recordings were made from two separate cells.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
