Anti Visinin-Like Protein 1 (VLP1) antibody ValidAbTM
Alternative names
Visinin Like Protein 1, VILIP-1, VSNL1
Host
Mouse
Clonality
Monoclonal
Target
VLP1
Description
Antibody to Visinin-Like Protein 1 (VLP1) - calcium sensor protein and marker of neuronal damage. Part of the ValidAb™ range of highly validated, data-rich antibodies.
VLP1 has no major isoforms other than the canonical sequence
Expression
Visinin-like protein 1 (VLP1) is found exclusively within the neurons of the nervous system, where it has abundant expression. While present throughout the nervous system, the specific levels of its expression vary between different neuronal populations with particularly strong expression in cerebellar granule cells.
Subcellular expression
VLP1 is localized to the neuronal cytosol during periods of low activity where Ca2+ concentrations are low but then translocates and associates with both membranes of organelles and the cell membrane during periods of high neuronal firing when Ca2+ is elevated
Target function
VLP1 functions primarily as a neuronal calcium sensor protein, equipped with a myristoylation-consensus sequence and four EF-hand motifs that enable a Ca2+-myristoyl switch for calcium-dependent membrane targeting and dimerization. This mechanism allows VLP1 to modulate intracellular signaling pathways, particularly in the central nervous system. In the brain, VLP1 is implicated in calcium-mediated neuronal injury and its elevated levels in cerebrospinal fluid serve as a biomarker for neuronal damage in conditions such as Alzheimer's disease, stroke, and general brain injury. Beyond the nervous system, VLP1 is expressed in various other tissues and notably acts as a target gene of the Wnt/β-catenin signaling pathway, contributing to apoptosis resistance in colorectal cancer cells, a role that also depends on its myristoylation, Ca2+-binding, and dimerization.
Post translational modifications
VLP1 is subject to removal of the initial methionine and there have been reports of ubiquitination on residues 18 and 63.
Homology (compared to human)
Mouse and rat VLP1 have 99.48% homology with human VLP1 due to a single base change (A to V at position 104)
Similar proteins
Hippocalcin-like protein 4 (HLP4) is the most similar human protein to VLP1 (89.5% identity) with other proteins having <70% similarity scores.
Storage & Handling
Storage instructions
-20°C
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
What guarantee do you have that my VLP1 antibody will perform as expected?
We guarantee that your VLP1 antibody will work for the applications and species we list on the datasheet. If the antibody fails to perform as expected then we are happy to offer a 100% refund guarantee. For more details please see our guarantee policy.
Will my VLP1 antibody work against species that have not been listed on the datasheet?
A species not being listed doesn’t mean that the antibody won’t work, just that we haven’t tested it. If you test one of our antibodies in a new species please let us know (positive or negative)!
What protocols are available for use with this VLP1 antibody
We have made a comprehensive collection of protocols that we have used in our experiments to validate this VLP1 antibody.
What counterstains do you recommend for use in ICC and IHC with this VLP1 antibody?
We recommend using either DAPI or Hoechst 33342 to label cell nuclei. In some experiments it is also helpful to label actin filaments in the cytoskeleton using a Phalloidin conjugate such as FITC Phalloidin or Rhodamine Phalloidin-TRITC.
What mounting media do you recommend to use with this antibody?
We recommend using one of our high performance mounting medias, supplied as either hardset or aqeous with a range of counterstains:
References for Anti Visinin-Like Protein 1 (VLP1) antibody ValidAbTM
References are publications that support the biological activity of the product
Visinin-like protein 1 levels in blood and CSF as emerging markers for Alzheimer's and other neurodegenerative diseases.
Halbgebauer S, Steinacker P, Riedel D, Oeckl P, Anderl-Straub S, Lombardi J, von Arnim CAF, Nagl M, Giese A, Ludolph AC, Otto M. (2022 ) Alzheimers Res Ther. :
Antibody to Visinin-Like Protein 1 (VLP1) - calcium sensor protein and marker of neuronal damage. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Western Blot Protocol (1 MB) 
