Prestained Protein Ladder (11 – 270 kDa)

(HB9198)
Technical documents: Datasheet

Product overview

Name Prestained Protein Ladder (11 – 270 kDa)
Biological description

Dual color protein broad range protein ladder used for estimated the molecular weight of unknown proteins in SDS-PAGE and Western Blotting. Contains 10 proteins of known molecular weights coupled to chromophores for easy visualisation.

Description

Dual color broad range protein ladder

Write Your Own Review
You're reviewing:Prestained Protein Ladder (11 – 270 kDa)
Rate this item:

Images

Prestained Protein Ladder (11 – 270 kDa)

5µL loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.

Comparison between Prestained Protein Ladder (11 – 270 kDa) and NEB P7718S.

5µL each of HB9198 and NEB P7718S were loaded onto a 4-20% Tris-glycine SDS-PAGE gel and run at 160V for approximately 60 minutes before being transferred to a PVDF membrane for 90 minutes at 400mA.

Biological Data

Application notes

We recommend loading between 2µl and 5µl per lane of a polyacrylamide gel. There is no need heat, reduce or dilute in sample buffer before use.

Solubility & Handling

Storage instructions

-20°C

Storage buffer

62.5 mM Tris-H3PO4 (pH 7.5), 2% SDS, 1 mM EDTA, 10 mM DTT, 1 mM sodium azide, 33% glycerol

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

=
x
x
More Info

Dilution

x
=
x
More Info

References for Prestained Protein Ladder (11 – 270 kDa)

References are publications that support the biological activity of the product
  • Western blot: technique, theory, and trouble shooting.

    Mahmood T et al (2012) North American journal of medical sciences 4 : 429-34

1 Item