Hello Bio Western Blot Stripping buffer allows the effective removal of primary and secondary antibodies from a PVDF or nitrocellulose membrane in order to facilitate subsequent reprobing of chemiluminescent Western blots. It protects membrane bound proteins and is quick and effective while not requiring use of a fume hood or elevated temperatures.Â
Description
Buffer for stripping of chemiluminscent Western blots
Figure 1. Stripping and re-probing of PVDF membrane using HB7756
An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with a β-actin antibody before being stripped with HB7756 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol
Figure 1. Stripping and re-probing of PVDF membrane using HB7756
An array of samples from different tissues were loaded onto an acrylamide gel before being transferred to PVDF. The membrane was probed with a β-actin antibody before being stripped with HB7756 following the protocol detailed in the application notes. The membrane was then successfully re-probed with HB9177. For more information on Western Blotting, please see our comprehensive Western blotting protocol
Biological Data
Application notes
Stripping Protocol
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Wash any remaining ECL solution off the membrane with a quick wash in PBS
Incubate twice for 10 minutes each in fresh stripping buffer
Wash membrane briefly in two changes of PBST (PBS with 0.1% Tween20)
Wash membrane 3 times in PBST for 5 minutes per wash