Anti-MAP2 antibody ValidAbTM
Product overview
Name | Anti-MAP2 antibody ValidAbTM |
Description | Antibody to MAP2 - cytoskeletal protein used as a neuronal marker. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
Host | Mouse |
Clonality | Monoclonal |
Target | MAP2 |
Validation data
Product information
Immunogen | Microtubule preparation derived from bovine brain and enriched for kinesin |
Epitope | Localised to within amino acids 1375 to 1395 (CPPAVSEADLATDERADVQME) |
Clone number | MT-07 |
Isotype | IgG1 |
Purification | Protein A affinity chromatography |
Concentration | 1mg/ml |
Formulation | Lyophilised. When reconstituted contains PBS with 15mM sodium azide and 1% recombinant BSA |
Predicted species reactivity | Human, Mouse, Pig, Cow |
Tested species reactivity | Mouse, Rat |
Tested applications
Applications | ICC, WB, IHC(IF) |
Western blot optimal concentration | 1µg/ml (1:1000) as measured in rat brain cytosol |
IHC(IF) optimal concentration | 1µg/ml (1:1000) as measured in rat hippocampal sections |
ICC optimal concentration | 1µg/ml (1:1000) as measured in cultured rat neurones |
Positive control | MAP2 should be found in any neural tissue sample but is not widely expressed in cell lines. |
Negative control | Non-neural tissues such as liver or muscle. Most common non-neural derived cell lines, such as HeLa and HEK293 are also MAP2 negative. |
Open data link | Please follow this link to OSF |
Target information
Other names | MAP-2, Microtubule-associated protein 2 |
UniProt ID | P11137 |
Structure image | |
Gene name | MAP2 |
NCBI full gene name | microtubule associated protein 2 |
Entrez gene ID |
Amino acids | 1827 (199.5kDa) |
Isoforms | MAP2 has 4 key isoforms: Isoform 1 (MAP2b), 1827aa, 199.5kDa; Isoform 2 (MAP2c), 471aa, 49.6kDa, missing aa152-1507 - juvenile isoform not expressed in adulthood; Isoform 3, 1823aa, 199.0kDa, missing aa152-155; Isoform 4, 559aa, 59.0kDa, multiple substitutions and missing aa230-1528. |
Expression | Expressed highly within the brain (neuron specific) and to a lesser degree in the testes |
Subcellular expression | Expressed as part of the cytoskeleton |
Processing | None |
Post translational modifications | MAP2 contains numerous phosphorylation sites however none occur with the epitope of HB9587 |
Homology (compared to human) | Mouse and rat show 79.8% and 77.7% identity to human MAP2 respectively in a BLAST search. |
Similar proteins | None |
Epitope homology (between species) | Human, 100% identity Mouse, 76.2% identity Rat, 71.4% identity |
Epitope homology (other proteins) | No significant homology with other proteins |
Storage & Handling
Storage instructions | -20°C then use reconstitution advice |
Reconstitution advice | We recommend reconstituting with either:
Take care when opening as the precipitate is extremely light and can easily be lost if disturbed. When reconstituting make sure that the antibody is thoroughly dissolved by pipetting up and down before giving the antibody a brief spin at <10,000g to make sure that all material is recovered and at the bottom of the tube. For more information please see our detailed guide on storing and using your antibody |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
References for Anti-MAP2 antibody ValidAbTM
-
The MAP2/Tau family of microtubule-associated proteins
Dehmelt L and Halpain S (2005) Genome Biol 6(1) : 204 -
Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons
Chen J et al (1992) Nature 360(6405) : 674-677 -
Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein
Lewis SA, Wang DH and Cowan NJ (1988) Science 242(4880) : 936-939 -
Differences in the cellular distributions of two microtubule-associated proteins, MAP1 and MAP2, in rat brain
Huber G, Matus A (1984) Journal of Neuroscience 4(1) : 151-160