Anti-GFP antibody ValidAb™
(HB8912)
Product overview
| Name | Anti-GFP antibody ValidAb™ |
| Description | Antibody to GFP - green coloured fluorescent protein widely used as a tag in molecular biology. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target | GFP |
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Validation data
Figure 1. pEGFP-C2 transfected HEK293 cells showing co-localised staining of EGFP and HB8912.
Figure 2. Specific HB8912 staining only in pEGFP-C2 transfected HEK293 cells.
Figure 3. Concentration response of HB8912 staining in pEGFP-C2 transfected HEK293 cells.
Figure 4. The effect of varying HB8912 concentration upon staining in pEGFP-C2 transfected HEK293 cells.
Figure 5. EGFP tagged Cannabinoid receptor 1 c-terminal domain (EGFP-ctCB1R) detected in virally transfected HEK293 cells using HB8912.
Figure 1. pEGFP-C2 transfected HEK293 cells showing co-localised staining of EGFP and HB8912.
Figure 2. Specific HB8912 staining only in pEGFP-C2 transfected HEK293 cells.
Figure 3. Concentration response of HB8912 staining in pEGFP-C2 transfected HEK293 cells.
Figure 4. The effect of varying HB8912 concentration upon staining in pEGFP-C2 transfected HEK293 cells.
Figure 5. EGFP tagged Cannabinoid receptor 1 c-terminal domain (EGFP-ctCB1R) detected in virally transfected HEK293 cells using HB8912.
Product information
| Immunogen | full length EGFP protein |
| Purification | Affinity purification using immunogen as ligand |
| Concentration | 1mg/ml |
| Formulation | Lyophilised. When reconstituted contains PBS with 15mM sodium azide and 1% recombinant BSA |
| Predicted species reactivity | NA |
| Tested species reactivity | NA |
Tested applications
| Applications | ICC, WB |
| Western blot optimal concentration | Dependent upon sample GFP expression. We used 100ng/ml (1:10,000 dilution) in pEGFP-C2 transfected HEK293 cells. |
| ICC optimal concentration | Dependent upon sample GFP expression. We used as low as 500ng/ml (1:2,000 dilution) in pEGFP-C2 transfected HEK293 cells. |
| Positive control | Any tissue or cell sample that has been engineered to express GFP. |
| Negative control | Any wild type tissue or cellular sample. |
| Open data link | Please follow this this link to OSF |
Target information
| Other names | EGFP, green fluorescent protein, EYFP |
| UniProt ID | P42212 |
| Structure image |  |
| Gene name | GFP |
| NCBI full gene name | green fluorescent protein |
| Amino acids | 238 (27kDa) |
| Isoforms | None |
| Expression | Exogenously expressed only. Not expressed natively in mammalian cells. |
| Subcellular expression | GFP is generally expressed cytosolically in basic constructs however expression can be directed to any cellular compartment through GFP-tagged proteins that naturally express in only certain compartments. |
| Processing | NA |
| Post translational modifications | NA |
| Homology (compared to human) | NA |
| Similar proteins | EGFP (enhanced GFP, 26.9kDa) and YFP (yellow fluorescent protein, 26.4kDa) are both extremely similar with HB8912 recognising these. |
Storage & Handling
| Storage instructions | -20°C then use reconstitution advice |
| Reconstitution advice | We recommend reconstituting with either:
Take care when opening as the precipitate is extremely light and can easily be lost if disturbed. When reconstituting make sure that the antibody is thoroughly dissolved by pipetting up and down before giving the antibody a brief spin at <10,000g to make sure that all material is recovered and at the bottom of the tube. For more information please see our detailed guide on storing and using your antibody |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
References for Anti-GFP antibody ValidAb™
References are publications that support the biological activity of the product
-
Green fluorescent protein: A perspective
Remington SJ (2011) Protein Science 20(9) : 1509-1519 -
Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes
Stepaneko O et al (2008) Curr Protein Pept Sci. 9(4) : 338-369 -
A guide to choosing fluorescent proteins
Shaner N, Steinbach P and Tsien R (2005) Nature Methods 2(12) : 905-909 -
The green fluorescent protein
Tsien RY (1998) Annu Rev Biochem 67 : 509-544 -
Crystal structure of the Aequorea victoria green fluorescent protein
Ormö M et al (1996) Science 273(5280) : 1392-1395
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