Product overview

Name Anti-GFP antibody ValidAb™
Description

Antibody to GFP - green coloured fluorescent protein widely used as a tag in molecular biology. Part of the ValidAb™ range of highly validated, data-rich antibodies.

Host Rabbit
Clonality Polyclonal
Target GFP
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Validation data

Figure 1. pEGFP-C2 transfected HEK293 cells showing co-localised staining of EGFP and HB8912.

Figure 2. Specific HB8912 staining only in pEGFP-C2 transfected HEK293 cells.

Figure 3. Concentration response of HB8912 staining in pEGFP-C2 transfected HEK293 cells.

Figure 4. The effect of varying HB8912 concentration upon staining in pEGFP-C2 transfected HEK293 cells.

Figure 5. EGFP tagged Cannabinoid receptor 1 c-terminal domain (EGFP-ctCB1R) detected in virally transfected HEK293 cells using HB8912.

Product information

Immunogen full length EGFP protein
Purification Affinity purification using immunogen as ligand
Concentration 1mg/ml
Formulation Lyophilised. When reconstituted contains PBS with 15mM sodium azide and 1% recombinant BSA
Predicted species reactivity NA
Tested species reactivity NA

Tested applications

Applications ICC, WB
Western blot optimal concentration Dependent upon sample GFP expression. We used 100ng/ml (1:10,000 dilution) in pEGFP-C2 transfected HEK293 cells.
ICC optimal concentration Dependent upon sample GFP expression. We used as low as 500ng/ml (1:2,000 dilution) in pEGFP-C2 transfected HEK293 cells.
Positive control

Any tissue or cell sample that has been engineered to express GFP.

Negative control

Any wild type tissue or cellular sample.

Open data link

Please follow this this link to OSF

Target information

Other names EGFP, green fluorescent protein, EYFP
UniProt ID P42212
Structure image Structure image
Gene name GFP
NCBI full gene name green fluorescent protein
Amino acids 238 (27kDa)
Isoforms None
Expression Exogenously expressed only. Not expressed natively in mammalian cells.
Subcellular expression GFP is generally expressed cytosolically in basic constructs however expression can be directed to any cellular compartment through GFP-tagged proteins that naturally express in only certain compartments.
Processing NA
Post translational modifications

NA

Homology (compared to human) NA
Similar proteins EGFP (enhanced GFP, 26.9kDa) and YFP (yellow fluorescent protein, 26.4kDa) are both extremely similar with HB8912 recognising these.

Storage & Handling

Storage instructions -20°C then use reconstitution advice
Reconstitution advice

We recommend reconstituting with either:


  • dH2O and storing at 4°C
  • 50:50 ratio of dH2O to glycerol and storing at -20°C
  • dH2O then aliquot and store at -80°C


Take care when opening as the precipitate is extremely light and can easily be lost if disturbed. When reconstituting make sure that the antibody is thoroughly dissolved by pipetting up and down before giving the antibody a brief spin at <10,000g to make sure that all material is recovered and at the bottom of the tube.


For more information please see our detailed guide on storing and using your antibody

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

References for Anti-GFP antibody ValidAb™

References are publications that support the biological activity of the product

FAQs

Any other questions?

For any other questions about our antibody products please see our technical FAQs for antibodies