Anti-MAP2 antibody ValidAbTM
(HB6581)
Product overview
| Name | Anti-MAP2 antibody ValidAbTM |
| Host | Chicken |
| Clonality | Polyclonal |
| Target | MAP2 |
| Description | Antibody to MAP2 - cytoskeletal protein used as a neuronal marker. Part of the ValidAb™ range of highly validated, data-rich antibodies. |
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Validation data
Figure 1. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 40x objective (1.28x zoom), 405nm (11.1% power, PMT: 716V gain) and 488nm (1% power, Hyd: 10% gain) lasers in a z-stack (0.44µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 2. GluN1 expression on a cultured cortical rat neuron using HB6581 MAP2 counterstaining.
HB6581 staining of MAP2 provides an excellent counterstain for visualisation of GluN1 stained with HB7535. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated and polyclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured as a tilescan using a Leica DMI6000 inverted epifluorescence microscope with a Photometrics Prime 95B camera. The image was captured using a 40x objective with DAP (40ms exposure), L5 (51ms exposure) and RHO (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 3. Colocalization of GluN1 and GluA1-4 in cultured cortical rat neurons.
Both GluN1 and GluA1-4 are expressed in cultured rat cortical neurons and are visualised using HB7535 (mouse monoclonal anti-GluN1) and HB7534 (rabbit polyclonal anti-GluA1-4) antibodies using HB6581 (chicken polyclonal anti-MAP2 antibody) counterstain. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1), HB7534 (rabbit polyclonal GluA1-4) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated, 1:300 dilution, polyclonal goat anti-rabbit DyLight 650 conjugated, 1:300 dilution and polyoclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 63x objective (2.01x zoom), 405nm (11.1% power, PMT: 543V gain), 488nm (1% power, Hyd: 10% gain), 561nm (1% power, Hyd: 10% gain) and 633nm (5% power, Hyd: 18.6% gain) laser lines in a z-stack (0.38µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 4. Concentration response of HB6581 staining in a rat brain cytosol preparation.
HB6581 shows strong affinity for MAP2 with bands being visible at dilutions as low as 1:32,000. A dual band is visible as this antibody recognises both MAP2a and MAP2b isoforms of MAP2. Method: Cytosol fractions were prepared from fresh rat brains following established protocols (Molnar et al., 1993. Neuroscience 53:307-326). Cytosol samples were loaded (20µg / lane) onto a 5% acrylamide gel alongside a protein ladder before being run at 60V for 30 minutes followed by 130V for 80 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. Following transfer, the membrane was cut into strips using Ponceau dye to visualise and cut individual lanes. Strips were blocked for 2hrs in 5% non-fat dry milk before being incubated overnight at 4°C in HB6581. Each strip was incubated separately with a separate HB6581 concentration with this ranging from 1:2000 dilution to a 1:512,000 dilution). Following washing the membrane was incubated in secondary antibody (1:10,000 dilution, Polyclonal goat anti-chicken HRP conjugated) for 2hrs. For more detail please see our Western blotting protocol. Detection was accomplished using Clarity Western ECL substrate (BioRad, 1705061) and a Licor Odyssey Fc imaging system (ECL channel: 8 min exposure, 700nm channel: 30 sec exposure). Band intensity was calculated using Image Studio version 5.2.5 (LiCor) and a graph was constructed in GraphPad Prism 9 using a 3-parameter Hill equation curve fit.
Figure 5. GluN1 expression on a cultured cortical rat neuron using HB6581 MAP2 labelling.
HB6581 staining of MAP2 provides an excellent counterstain for visualisation of GluN1 stained with HB7535. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated and polyclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured as a tilescan using a Leica DMI6000 inverted epifluorescence microscope with a Photometrics Prime 95B camera. The image was captured using a 40x objective with DAP (40ms exposure), L5 (80ms exposure) and RHO (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 6. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 63x objective (1.28x zoom), 405nm (11.1% power, PMT: 543V gain) and 488nm (1% power, Hyd: 10% gain) laser lines in a z-stack (0.38µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 7. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. The image was captured using a 40x objective with DAP (40ms exposure) and L5 (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 1. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 40x objective (1.28x zoom), 405nm (11.1% power, PMT: 716V gain) and 488nm (1% power, Hyd: 10% gain) lasers in a z-stack (0.44µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 2. GluN1 expression on a cultured cortical rat neuron using HB6581 MAP2 counterstaining.
HB6581 staining of MAP2 provides an excellent counterstain for visualisation of GluN1 stained with HB7535. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated and polyclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured as a tilescan using a Leica DMI6000 inverted epifluorescence microscope with a Photometrics Prime 95B camera. The image was captured using a 40x objective with DAP (40ms exposure), L5 (51ms exposure) and RHO (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 3. Colocalization of GluN1 and GluA1-4 in cultured cortical rat neurons.
Both GluN1 and GluA1-4 are expressed in cultured rat cortical neurons and are visualised using HB7535 (mouse monoclonal anti-GluN1) and HB7534 (rabbit polyclonal anti-GluA1-4) antibodies using HB6581 (chicken polyclonal anti-MAP2 antibody) counterstain. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1), HB7534 (rabbit polyclonal GluA1-4) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated, 1:300 dilution, polyclonal goat anti-rabbit DyLight 650 conjugated, 1:300 dilution and polyoclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 63x objective (2.01x zoom), 405nm (11.1% power, PMT: 543V gain), 488nm (1% power, Hyd: 10% gain), 561nm (1% power, Hyd: 10% gain) and 633nm (5% power, Hyd: 18.6% gain) laser lines in a z-stack (0.38µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 4. Concentration response of HB6581 staining in a rat brain cytosol preparation.
HB6581 shows strong affinity for MAP2 with bands being visible at dilutions as low as 1:32,000. A dual band is visible as this antibody recognises both MAP2a and MAP2b isoforms of MAP2. Method: Cytosol fractions were prepared from fresh rat brains following established protocols (Molnar et al., 1993. Neuroscience 53:307-326). Cytosol samples were loaded (20µg / lane) onto a 5% acrylamide gel alongside a protein ladder before being run at 60V for 30 minutes followed by 130V for 80 minutes. Wet transfer to a PVDF membrane was completed in 90 minutes using 400mA. Following transfer, the membrane was cut into strips using Ponceau dye to visualise and cut individual lanes. Strips were blocked for 2hrs in 5% non-fat dry milk before being incubated overnight at 4°C in HB6581. Each strip was incubated separately with a separate HB6581 concentration with this ranging from 1:2000 dilution to a 1:512,000 dilution). Following washing the membrane was incubated in secondary antibody (1:10,000 dilution, Polyclonal goat anti-chicken HRP conjugated) for 2hrs. For more detail please see our Western blotting protocol. Detection was accomplished using Clarity Western ECL substrate (BioRad, 1705061) and a Licor Odyssey Fc imaging system (ECL channel: 8 min exposure, 700nm channel: 30 sec exposure). Band intensity was calculated using Image Studio version 5.2.5 (LiCor) and a graph was constructed in GraphPad Prism 9 using a 3-parameter Hill equation curve fit.
Figure 5. GluN1 expression on a cultured cortical rat neuron using HB6581 MAP2 labelling.
HB6581 staining of MAP2 provides an excellent counterstain for visualisation of GluN1 stained with HB7535. Method: neurones were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB7535 (mouse monoclonal anti-GluN1) and HB6581 (chicken polyclonal anti-MAP2) antibodies were incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with secondary antibodies (Polyclonal goat anti-chicken Alexa Fluor 488 conjugated and polyclonal goat anti-mouse DyLight 550 conjugated, 1:300 dilution). DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured as a tilescan using a Leica DMI6000 inverted epifluorescence microscope with a Photometrics Prime 95B camera. The image was captured using a 40x objective with DAP (40ms exposure), L5 (80ms exposure) and RHO (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 6. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. Images were captured using a Leica SP8 AOBS confocal laser scanning microscope attached to a Leica DM I8 inverted epifluorescence microscope. The image was captured in Lightning deconvolution mode using a 63x objective (1.28x zoom), 405nm (11.1% power, PMT: 543V gain) and 488nm (1% power, Hyd: 10% gain) laser lines in a z-stack (0.38µm spacing). The stack was flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Figure 7. Cultured rat neuron labelled for MAP2 with HB6581
MAP2 staining using HB6581 reveals the complex morphology of a cultured rat cortical neuron. Method: neurons were cultured from E17-E18 rat embryos following established protocols (Martin and Henley, 2004. EMBO, 4749–4759) and fixed with 4% PFA on DIV21. Antigen retrieval was carried out by heating the coverslip to 95°C in 100mM Tris, 5% urea, pH9.5. Following washes, cells were permeabilised with 0.1% Triton X-100 followed by blocking in 1% BSA, 300mM glycine. HB6581 (chicken polyclonal anti-MAP2) was incubated overnight (4°C) at a 1:1,000 dilution. This was followed by a one-hour incubation with a polyclonal goat anti-chicken Alexa Fluor 488 conjugated secondary antibody at a 1:300 dilution. DAPI (HB0747) was used at 1µg/ml to visualise cell nuclei. For more detail please see our ICC protocol. The image was captured using a 40x objective with DAP (40ms exposure) and L5 (80ms exposure) filters. Following capture, the image was deconvolved using Huygens software before having the stack flattened using a maximum Z projection in ImageJ (Schindelin et al., 2012. Nat Methods, 9(7), 676–682).
Product information
| Immunogen | Combination of three recombinant proteins derived from human MAP2 (aa233-684, aa712-1136 and aa1137 - 1588) |
| Isotype | IgY |
| Purification | Affinity chromatography |
| Formulation | Lyophilised. When reconstituted contains IgY preparation with 5mM sodium azide and 1% recombinant BSA. |
| Predicted species reactivity | Mouse, Rat, Human |
| Tested species reactivity | Mouse, Rat |
Tested applications
| Applications | ICC |
| ICC optimal concentration | 1:2,000 as tested in cultured rat neurons |
| Positive control | MAP2 should be found in any neural tissue sample but is not widely expressed in cell lines. |
| Negative control | Non-neural tissues such as liver or muscle. Most common non-neural derived cell lines, such as HeLa and HEK293 are also MAP2 negative. |
| Open data link | Please follow this link to OSF |
Target information
| Other names | MAP-2, Microtubule-associated protein 2 |
| UniProt ID | P11137 |
| Structure image |  |
| Gene name | MAP2 |
| NCBI full gene name | microtubule associated protein 2 |
| Entrez gene ID |
| Amino acids | 1827 (199.5kDa) |
| Isoforms | MAP2 has 4 key isoforms: Isoform 1 (MAP2b), 1827aa, 199.5kDa; Isoform 2 (MAP2c), 471aa, 49.6kDa, missing aa152-1507 - juvenile isoform not expressed in adulthood; Isoform 3, 1823aa, 199.0kDa, missing aa152-155; Isoform 4, 559aa, 59.0kDa, multiple substitutions and missing aa230-1528. |
| Expression | Expressed highly within the brain (neuron specific) and to a lesser degree in the testes |
| Subcellular expression | Expressed as part of the cytoskeleton |
| Processing | None |
| Post translational modifications | MAP2 contains numerous phosphorylation sites which overlap with the immunogen sequence. |
| Homology (compared to human) | Mouse and rat show 79.8% and 77.7% identity to human MAP2 respectively in a BLAST search. |
| Similar proteins | None |
Storage & Handling
| Storage instructions | -20°C then use reconstitution advice |
| Reconstitution advice | We recommend reconstituting with either:
Take care when opening as the precipitate is extremely light and can easily be lost if disturbed. When reconstituting make sure that the antibody is thoroughly dissolved by pipetting up and down before giving the antibody a brief spin at <10,000g to make sure that all material is recovered and at the bottom of the tube. For more information please see our detailed guide on storing and using your antibody |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Technical guides
Western Blot Protocol (1 MB) Immunocytochemistry Protocol (1.2 MB) Immunohistochemistry Protocol (1.6 MB) References for Anti-MAP2 antibody ValidAbTM
References are publications that support the biological activity of the product
-
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L et al (2005) Genome biology 6 : 204 -
Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons.
Chen J et al (1992) Nature 360 : 674-7 -
Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein.
Lewis SA et al (1988) Science (New York, N.Y.) 242 : 936-9 -
Differences in the cellular distributions of two microtubule-associated proteins, MAP1 and MAP2, in rat brain.
Huber G et al (1984) The Journal of neuroscience : the official journal of the Society for Neuroscience 4 : 151-60
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