Anti-NeuN antibody ValidAb™

N-terminal 100 amino acids of human FOX3 expressed and purified from E. coli

Immunogen affinity purification

1:1000 (1μg/ml) as assessed in rat brain sections

NeuN is highly expressed in the neurons of the CNS and PNS. It is also expressed in SH-SY5Y cells.

Any tissue not of neural origin. Most cell lines are NeuN negative.

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FOX3, RNA binding protein fox-1 homolog 3, Fox-1 homolog C, RBFOX3, RFOX3

146713

Dependent on isoform

NeuN binds primarily to FOX3 which has two isoforms. Isoform 1 is described as the canonical sequence with 312 amino acids (33.8kDa) while isoform 2 has a 13 residue insert at position 312 leading to a total length of 325 amino acids (35.1kDa). NeuN antibodies also bind to synapsin-1 in western blot experiments (but not in IHC or ICC) which has two isoforms. Isoform 1 is 705aa long (74.1kDa) while isoform 2 is shorter at 669aa (70.0kDa).

NeuN is expressed only within neurones. While the vast majority of neurones express NeuN some cell types such as Purkinje cells, stellate and golgi cells do not show immunoreactivity.

Expression is primarily localised to the nucleus however some FOX3 isoforms can localise to the cytosol.

FOX3 is a splicing regulator of pre-mRNA responsible for neuronal specific alternative splicing of neuronal proteins.

None

Phosphorylation has been reported (see Lind et al., 2004. J Neurosci Res. 79: 295-302) which is directly related to immunoreactivity whereby dephosphorylation abolished staining.

Mouse FOX3 shows 95.02% identity to human FOX3 wheras rat FOX3 shows no similarity due to a large 47 residue insertion at amino acid 252 in rats.

RNA-binding protein fox-1 homolog 1 (40-44kDa) shows 67.3% identity while RNA-binding protein fox-1 homolog 2 (37-47kDa) shows 56.5% identity.

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