Stem cell research is a dynamic, rapidly expanding field, representing one of the most exciting areas in life science. Hello Bio offer a range of high quality, low-cost small molecules for use in various stem cell culture techniques:
Embryonic and Induced Pluripotent Stem Cells (ESCs and iPSCs)
Neural Stem Cells (NSCs)
Organoids and 3D cell culture
Mesenchymal Stem Cells (MSCs)
Cell Culture Reagents & Buffers
Stem cells are unspecialized cells that have the ability to undergo self-renewal through mitotic cell division or differentiate into specialized cell types. Stem cells derived from distinct developmental stages may display different developmental potential. For example, pluripotent stem cells (PSCs) can give rise to all cell types of the entire embryo but not the extraembryonic tissues, such as placenta.
Types of stem cells:
Embryonic Stem Cells (ESCs)
Pluripotent cells found in the inner cell mass of blastocysts
Induced Pluripotent Stem Cells (iPSCs)
Pluripotent cells produced by the reprogramming of somatic cells
Haematopoietic Stem Cells (HSCs)
Multipotent cells that can develop into all types of blood cells, including myeloid-lineage and lymphoid-lineage cells. Found in several organs, such as peripheral blood, bone marrow and umbilical cord blood.
Mesenchymal Stem Cells (MSCs)
Multipotent stromal cells able to differentiate into a variety of cell types, including osteoblasts, chondrocytes, myocytes and adipocytes.
Neural Stem Cells (NSCs)
Multipotent cells in the nervous system that can self-renew and give rise to differentiated lineages of neurons and glial cells, including oligodendrocytes and astrocytes.
Cancer Stem Cells (CSCs)
Subset of cells found in tumors, characterized by self-renewal and continuous proliferation, leading to tumorigenesis, metastasis, and maintenance of tumor heterogeneity
Hello Bio offer a range of small molecules for use in a variety of stem cell culture techniques:
Hello Bio offer a range of high quality yet affordable tools for use in stem cell culture including:
Stem cell differentiation involves transformation of a cell into a more specialized cell type.
PSCs can be differentiated into specific cell types through the use of small molecules which modulate key signaling pathways which control proliferation and differentiation.
PSCs are increasingly being differentiated into specialized cell types for a wide range of applications, such as disease modeling, drug discovery, toxicity screening and cell therapy1.
An organoid is a self-organized three-dimensional (3D) structure that is typically derived from stem cells (pluripotent, fetal or adult), and mimics the key functional, structural and biological complexity of an organ.
To date, organoid cultures have been described for a variety of tissues, including intestinal2, liver3, pancreas4, kidney5, prostate6, lung7, optic cup8 and brain9.
Organoids provide stable, accessible and physiologically relevant models that recapitulate key elements of the modelled tissue.
Reprogramming typically refers to regression of a specialized cell to a simpler state, resulting in cells with pluripotent properties.
Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cell (PSC) that can be obtained by reprogramming somatic cells through the artificial expression of key transcription factors under specific culture conditions. Specialized cells may also be reprogrammed directly into another cell type, a process termed trans-differentiation.
Cellular proliferation describes increase in cell number resulting from cell division into two identical daughter cells, also termed as 'self-renewal.'
Maintenance of high quality human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is critical to success in all applications of PSC research. Specific culture conditions are required in order to maintain undifferentiated ES and iPS cells that are capable of self-renewal.
Neural stem cells (NSCs) are a subtype of progenitor cells in the nervous system that can self-renew and give rise to differentiated lineages of neurons and glial cells, including oligodendrocytes and astrocytes.
NSCs are present during neural development and also in the adult brain mainly in two neurogenic regions: the hippocampus and the subventricular zone (SVZ). The stem cell niche architecture allows adult neural stem cells to continuously generate neurons in the brain throughout life.
Neural conversion of PSCs can be achieved through dual SMAD inhibition, for example through combined use of LDN193189 and SB43153210.
