Product overview

Name CHIR 99021
Alternative names CHIR99021
Purity >98%
Description Potent, selective GSK3 inhibitor and Wnt signaling activator. Commonly used in organoid production and involved in reprogramming MEFs to IPSCs and fibroblasts to mature neurons.
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Biological Data

Biological description

Potent, selective and ATP-competitive GSK-3 inhibitor (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively).

Wnt signaling activator which is commonly used with PD 032501 as part of the 2i inhibitor combination.

Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over >20 other enzymes and receptors.

Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.

Commonly used in organoid production and also involved in reprogramming of fibroblasts to mature neurons.

Water soluble CHIR 99021 trihydrochloride also available.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO (20mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Purity >98%
Chemical name 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5 -methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
Molecular Weight 465.34
Chemical structure CHIR 99021  [252917-06-9] Chemical Structure
Molecular Formula C22H18Cl2N8
CAS Number 252917-06-9
PubChem identifier 9956119
InChi InChI=1S/C22H18Cl2N8/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32)
MDL number MFCD11846251

References for CHIR 99021

References are publications that support the biological activity of the product
  • The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.

    Li C et al (2013) PLoS One 8(12) : e84659.
  • Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

    Ye S et al (2012) PLoS One 7(4) : e35892.
  • Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.

    Li W et al (2009) Stem cells 27(12) : 2992-3000

3 Item(s)

These publications cite the use of CHIR 99021 purchased from Hello Bio:
  • CRISPR activation of endogenous genes reprograms fibroblasts into cardiovascular progenitor cells for myocardial infarction therapy

    Jiang L et al (2022) Mol Ther 30(1) : 54-74
    PubMedID: 34678511

1 Item