Description Potent, selective TGF-βRI ALK5, ALK4, ALK7 inhibitor. Induces sheet formation, proliferation, differentiation of ESC-derived endothelial cells and replaces SOX2 in reprogramming protocols.3D growth matrix component and can be used in production of organoids. Biological Data
Potent and selective transforming growth factor β type I receptor (TGF-βRI) actvin receptor-like kinase ALK5, ALK4 and ALK7 inhibitor (IC
50 = 94 nM at ALK5).
Specifically inhibits Smad2/3 activation and blocks TGF-β signal transduction.
Maintains the undifferentiated state of mouse embryonic stem cells (ESCs).
Also induces sheet formation, proliferation, differentiation of ESC-derived endothelial cells.
It additionally can be used as a 3D growth matrix component and can be used in production of organoids (e.g. brain/ blood vessel organoids).
It can also be used to replace the SOX2 factor in reprogramming protocols.
Solubility & Handling
Storage instructions Room temperature
Solubility overview Soluble in DMSO (100mM) and ethanol (10mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. Chemical Data
Chemical name 4-[4-(1,3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]-benzamide
Molecular Formula C 22H 16N 4O 3
PubChem identifier 4521392
InChiKey FHYUGAJXYORMHI-UHFFFAOYSA-N References for SB 431542
References are publications that support the biological activity of the product
Mechanism of SB431542 in inhibiting mouse embryonic stem cell differentiation. Du et al (2014) Cell Signal 26(10) : 2107-16 TGF-beta receptor kinase inhibitor enhances growth and integrity of embryonic stem cell-derived endothelial cells. Watabe et al (2003) J Cell Biol 163(6) : 1303-11 Inhibition of transforming growth factor (TGF)-beta1-induced extracellular matrix with a novel inhibitor of the TGF-beta type I receptor kinase activity: SB-431542. Laping et al (2002) Mol Pharmacol 62(1) : 58-64 SB-431542 is a potent and specific inhibitor of transforming growth factor-beta superfamily type I ALK receptors ALK4, ALK5, and ALK7. Inman et al (2002) Mol Pharmacol 62(1) : 65-74
These publications cite the use of SB 431542 purchased from Hello Bio:
Modeling early neuronal development in Kabuki Syndrome using human induced pluripotent stem cells. Kilpinen et al (2022) Thesis Epithelial folding of alveolar cells derived from human induced pluripotent stem cells on artificial basement membrane. Rofaani E et al (2022) Acta biomaterialia In vitro modelling of human microglial alterations associated with Alzheimer's disease and polygenic risk Salis et al (2022) Cardiff University : Thesis