Smo receptor agonist that activates the Hedgehog (Hh) signaling pathway to induce osteogenesis in multipotent mesenchymal progenitor cells. Also promotes differentiation of pre-adipocytes and myoblasts into osteoblasts when use in combination with BMP-4.
Frequently used to differentiate pluripotent stem cells (e.g. ESCs and iPSCs) to neural stem cells and defined neuronal populations such as motor neurons and midbrain dopaminergic neurons. Protocols often use puromorphamine in combination with CHIR99021 (CHIR), SB431542 (SB), LDN193189 (LDN), XAV939, Y-27632, DAPT, BDNF, GDNF, retinoic acid (RA) and forskolin).
Solubility & Handling
Solubility overview
Soluble in DMSO (100mM)
Storage instructions
-20°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
![Purmorphamine | [483367-10-8] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb3412.png "Purmorphamine | [483367-10-8]")
Understanding purity and quality - a guide for life scientists
