GDNF is widely expressed in many neuronal and non-neuronal tissues and is involved in multiple processes. It has been shown to enhance survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
GDNF promotes recovery of the injured nigrostriatal dopamine system and improves motor functions in rodent and nonhuman primate models of Parkinson's disease.
GDNF, BDNF and other supplements are often used to differentiate hPSC-derived neural progenitor cells into neurons.
Alternative names
Recombinant Human Glial-Derived Neurotrophic Factor, ATF1, ATF2, HFB1-GDNF, GDNF.
Purity
>95%
Description
Neurotrophin which enhances differentiation and survival of dopaminergic neurons
ED50 = < 0.1 ng/ml (determined by the proliferation of rat C6 cells), corresponding to a specific activity of >1.0x107units/mg.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Understanding purity and quality - a guide for life scientists
