γ-secretase inhibitor and classical notch inhibitor. Induces neuronal differentiation. 3D Growth matrix component and used in cerebral organoid differentiation media.
Figure 1. N2a cells and primary cultured cortical neurons treated with DAPT
N2a cells (left) and primary cultures cortical neurons (right) were treated with DAPT (HB3345) at indicated concentrations for 24 hours. In N2a cells, the left 3 lanes are transferred with empty vector. The right 3 lanes were transferred with APP Swedish plasmid. Full length APP is used to blot the gel. In neurons, there was no transfection. Instead the effect on endogenous APP is investigated. The same antibody of full length APP is used to detect the protein. Source: Customer data, published 13/01/20
Figure 1. N2a cells and primary cultured cortical neurons treated with DAPT
N2a cells (left) and primary cultures cortical neurons (right) were treated with DAPT (HB3345) at indicated concentrations for 24 hours. In N2a cells, the left 3 lanes are transferred with empty vector. The right 3 lanes were transferred with APP Swedish plasmid. Full length APP is used to blot the gel. In neurons, there was no transfection. Instead the effect on endogenous APP is investigated. The same antibody of full length APP is used to detect the protein. Source: Customer data, published 13/01/20
Biological Data
Biological description
Overview
DAPT is a γ-secretase inhibitor which blocks notch signaling. It is a classical notch inhibitor.
It has been reported to inhibit amyloid beta (Aβ) in a dose-dependent manner although at low doses, an increase in Aβ production in cell culture and animal models has also been reported.
Uses & applications
DAPT is used in a variety of stem cell applications.
It has been shown that in combination with other small molecules that inhibit SMAD (SB 431542, LDN193189), activate WNT (CHIR99021) and inhibit FGF-signaling (SU5402), DAPT yields 75% post-mitotic neurons in 11 days of differentiation. These are defined as peripheral sensory neurons.
Recently, DAPT in combination with LDN193189, SB431542, XAV939, PD0325901 and SU5402 has been shown to accelerate induction of early-born cortical neurons that have mature electrophysiological properties by day 16 of differentiation.
DAPT also enhances neuronal differentiation in ESC-derived embryoid bodies independent of Hh-signaling.
Additionally, the compound promotes cardiac differentiation of murine pluripotent stem cells and enhances the reprogramming of mouse fibroblasts to iCLMs (induced cardiac-like myocytes).
It is also used as a 3D Growth matrix component and used in cerebral organoid differentiation media.
Solubility & Handling
Storage instructions
+4°C
Solubility overview
Soluble in DMSO (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling.
Crawford and Roelink (2007) Dev Dyn 236(3) : 886-92
γ-secretase inhibitor and classical notch inhibitor. Induces neuronal differentiation. 3D Growth matrix component and used in cerebral organoid differentiation media.
![DAPT [208255-80-5] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb3345.png "DAPT [208255-80-5]")
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