|Name||Recombinant human BDNF protein|
|Biological description|| |
BDNF (Brain-derived neurotrophic factor) is a member of the neurotrophin growth factor family and is highly expressed in the brain. BDNF activates the TrkB and p75 (P75NTR) neurotrophin receptors.
BDNF, GDNF and other supplements are often used to differentiate hPSC-derived neural progenitor cells into neurons.
BDNF (human) is a recombinant protein expressed in and extracted from E. coli and purified to homogeneity.
|Alternative names||Brain-derived neurotrophic factor, MGC34632|
|Description||Member of the neurotrophin growth factor family. Plays an important role in synaptic plasticity and neurogenesis.|
Recombinant human BDNF protein product vial image | Hello Bio
|Application notes||ED50 = 1.3-2 µg/ml (determined by the dose-dependent induction of C6 cells proliferation)|
Solubility & Handling
|Solubility overview||Soluble in sterile deionized water (not less than 100 µg/ml)|
|Important||This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use|
|Chemical name||Brain-derived neurotropic factor human recombinant produced in E. Coli. Homodimer, non-glycosylated polypeptide containing 2 x 119 amino acids and an N-terminal Met. Total molecular mass ~27000. Purified by proprietary chromatography methods.|
|Sequence (one letter)||MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR|
|Formulation||Sterile filtered, white lyophilized powder (protein lyophilized without any additives)|
|Protein length||2 x 119 AA|
References for Recombinant human BDNF protein
The ability of BDNF to modify neurogenesis and depressive-like behaviors is dependent upon phosphorylation of tyrosine residues 365/367 in the GABA(A)-receptor γ2 subunit.Vithlani et al (2013) J Neurosci 33(39) : 15567-77
BDNF function in adult synaptic plasticity: the synaptic consolidation hypothesis.Bramham and Messaoudi (2005) Prog Neurobiol 76(2) : 99-125
Brain-derived neurotrophic factor.Binder and Scharfman (2004) Growth Factors 22(3) : 123-31
Neurotrophins: roles in neuronal development and function.Huang and Reichardt (2001) Annu Rev Neurosci 24 : 677-736
Cultured hippocampal neurons show responses to BDNF, NT-3, and NT-4, but not NGF.Ip et al (1993) J Neurosci 13(8) : 3394-405
BDNF-induced BDNF release mediates presynaptic LTP and is regulated by cannabinoidsCastillo et al (2021) bioRxiv https://doi.org/10.1101/2021.12.30.474558 : doi
Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT).Romero-Leguizamón et al (2019) Sci Rep. 6(1) : 1486PubMedID: 30728375