Selective, competitive NMDA receptor antagonist
|Biological description|| |
Widely used, selective and competitive NMDA receptor antagonist which binds at the glutamate site. It is the more active form of DL-AP5.
|Alternative names||2-APV, D-APV|
|Customer comments|| |
I made the discovery that the NMDA receptor is the trigger for the induction of LTP using D-AP5 synthesized by Jeff Watkins, the discoverer of the NMDA receptor... I now obtain my D-AP5 from Hello Bio. I love their products and ethos and that is why I accepted a position on their Scientific Advisory Board.
Professor Graham Collingridge, winner of The Brain Prize, 2016
My lab used D-AP5 from Hello Bio and were very happy with it. It behaved exactly as expected! Professor Kei Cho, Chair of Neuroscience, University of Bristol, UK (Hello Bio Scientific Advisory Board Member)
My lab is very satisfied with your D-AP5 quality and price. Verified customer, European Brain Research Institute (EBRI)
I used to buy D-AP5 from another company, but Hello Bio is far more cost-effective and works great in our experiments. Verified customer, University of South Carolina
The D-AP5 works as expected, great price. Verified customer, UCSF
|Application notes|| |
The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 μM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 μM and reduces NMDA currents at lower concentrations of 1 and 10 μM (see Fig 1 above).
#Protocol 1: Evoked NMDA receptor currents
Solubility & Handling
|Storage instructions||Room temperature|
|Solubility overview||Soluble in water (100mM)|
|Important||This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.|
|Chemical name||D-(-)-2-Amino-5-phosphonopentanoic acid|
References for D-AP5
NMDA receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning.Morris RG et al (2013) Eur J Neurosci 37(5) : 700-17.
Actions of D and L forms of 2-amino-5-phosphonovalerate and 2-amino-4-phosphonobutyrate in the cat spinal cord.Davies J et al (1982) Brain Res 235(2) : 378-86.
Effects of pre or posttraining dorsal hippocampus D-AP5 injection on fear conditioning to tone, background, and foreground context.Schenberg EE et al (2008) Hippocampus 18(11) : 1089-93.
Age-dependent hippocampal network dysfunction in a mouse model of alpha-synucleinopathyTweedy et al (2018) Thessis : University of Newcastle
Calcium-Permeable AMPA Receptors Mediate the Induction of the Protein Kinase A-Dependent Component of Long-Term Potentiation in the Hippocampus.Park et al (2016 ) J Neurosci 36(2) : 622-31PubMedID: 26758849
SALM5 trans-synaptically interacts with LAR-RPTPs in a splicing-dependent manner to regulate synapse development.Choi et al (2016) Sci Rep 26 : 6:26676PubMedID: 27225731
mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.Valentinova and Mameli (2016) Cell Rep 16(9) : 2298-307PubMedID: 27545888
Structural basis of subunit selectivity for competitive NMDA receptor antagonists with preference for GluN2A over GluN2B subunits.Lind et al (2017) Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1707752114 : Epub ahead of printPubMedID: 28760974
NMDA receptors contribute to retrograde synaptic transmission from ganglion cell photoreceptors to dopaminergic amacrine cellsLiu et al (2017) Frontier in Cellular Neuroscience 11:279 : (1-13)