Selective, competitive NMDA receptor antagonist. Inhibits NMDAR-synaptic plasticity.
|Alternative names||2-APV, D-APV|
|Customer comments|| |
I made the discovery that the NMDA receptor is the trigger for the induction of LTP using D-AP5 synthesized by Jeff Watkins, the discoverer of the NMDA receptor... I now obtain my D-AP5 from Hello Bio. I love their products and ethos and that is why I accepted a position on their Scientific Advisory Board.
Professor Graham Collingridge, winner of The Brain Prize, 2016
My lab used D-AP5 from Hello Bio and were very happy with it. It behaved exactly as expected! Professor Kei Cho, Chair of Neuroscience, University of Bristol, UK (Hello Bio Scientific Advisory Board Member)
My lab is very satisfied with your D-AP5 quality and price. Verified customer, European Brain Research Institute (EBRI)
I used to buy D-AP5 from another company, but Hello Bio is far more cost-effective and works great in our experiments. Verified customer, University of South Carolina
The D-AP5 works as expected, great price. Verified customer, UCSF
|Biological description|| |
Widely used, selective and competitive NMDA receptor antagonist which binds at the glutamate site. It is the more active form of DL-AP5.
|Application notes|| |
#Figure 1: D-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 μM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 μM and reduces NMDA currents at lower concentrations of 1 and 10 μM (see Fig 1 above).
#Protocol 1: Evoked NMDA receptor currents
#Figure 2: D-AP5 inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 pyramidal neurones.
D-AP5 is commonly used to inhibit NMDA mediated synaptic events such as dendritic plateau potentials. Figure 2 shows that D-AP5 from Hello Bio completely abolishes plateau potential formation at 50µM (see Fig 2 above).
#Protocol 2: Inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 Pyramidal neurones
Solubility & Handling
|Storage instructions||Room temperature|
|Solubility overview||Soluble in water (100mM)|
|Important||This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.|
|Chemical name||D-(-)-2-Amino-5-phosphonopentanoic acid|
References for D-AP5
Age-dependent hippocampal network dysfunction in a mouse model of alpha-synucleinopathyTweedy et al (2018) Thessis : University of Newcastle
NMDA receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning.Morris RG et al (2013) Eur J Neurosci 37(5) : 700-17.
Effects of pre or posttraining dorsal hippocampus D-AP5 injection on fear conditioning to tone, background, and foreground context.Schenberg EE et al (2008) Hippocampus 18(11) : 1089-93.
Actions of D and L forms of 2-amino-5-phosphonovalerate and 2-amino-4-phosphonobutyrate in the cat spinal cord.Davies J et al (1982) Brain Res 235(2) : 378-86.
Microglial pattern recognition via IL-33 promotes synaptic refinement in developing corticothalamic circuits in mice.Han RT et al (2023) The Journal of experimental medicine 220PubMedID: 36520518
The selective dopamine D1 receptor agonist SKF81297 modulates NMDA receptor currents independently of D1 receptorsNesbit MO et al (2022) Neuropharmacology 207 : 108967PubMedID: 35077763
Edinger-Westphal peptidergic neurons enable maternal preparatory nestingTopilko T et al (2022) NeuronPubMedID: 35123655
Transient developmental increase in cortical projections to amygdala GABAergic neurons contribute to circuit dysfunction following early life stressLauri et al (2022) Biorxiv : https://doi.org/10.1101/2022.04.21.489031
GRID1/GluD1 homozygous variants linked to intellectual disability and spastic paraplegia impair mGlu1/5 receptor signaling and excitatory synapsesUng et al (2022) medRxiv : https://doi.org/10.1101/2022.05.16.22274994