CNQX disodium salt

(HB0205)
Technical documents: SDS CoA Datasheet

Product overview

Name CNQX disodium salt
Description Potent, competitive AMPA / kainate receptor antagonist. Disodium salt.
Purity >98%
Customer comments

The CNQX is going fine ! Verified customer, IBPS, Inserm, CNRS

It works exactly as it should! Dissolved in water, kept in aliquots in -20 freezer. Verified customers, SickKids (University of Toronto)

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CNQX disodium inhibition of AMPA receptor mediated EPSCs
CNQX disodium salt: Scientist Approved
CNQX disodium salt - vial

Biological Data

Biological description

CNQX disodium salt is a water soluble, potent and competitive AMPA and kainate receptor antagonist. CNQX also antagonizes NMDA receptors at the glycine site.


CNQX increases GABAA receptor spontaneous postsynaptic currents (sPSCs) and also shows neuroprotective actions.


CNQX also available.

Application notes

The AMPA receptor antagonist CNQX disodium salt is commonly used at concentrations of 10 μM to inhibit the actions of glutamate acting on AMPARs.

CNQX disodium salt from Hello Bio reduces both spontaneous and evoked EPSCs in cortical neurons at concentrations of 1 μM with full AMPA receptor blockade at 10 μM (see Fig 1 above).

 

#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of CNQX disodium salt until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of CNQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions Room temperature (desiccate)
Solubility overview Soluble in water (20mM)
Handling Hydroscopic solid, contact with air may cause material to change colour and become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 6-Cyano-7-nitroquinoxaline-2,3-dione disodium
Molecular Weight 276.12
Chemical structure CNQX disodium salt  [479347-85-8] Chemical Structure
Molecular Formula C9H2N4O4Na2
CAS Number 479347-85-8
PubChem identifier 2821
SMILES C1=C(C(=CC2=C1N=C(C(=N2)[O-])[O-])[N+](=O)[O-])C#N.[Na+].[Na+]
Source Synthetic
InChi InChI=1S/C9H4N4O4.2Na/c10-3-4-1-5-6(2-7(4)13(16)17)12-9(15)8(14)11-5;;/h1-2H,(H,11,14)(H,12,15);;/q;2*+1/p-2
InChiKey YCXDDPGRZKUGDG-UHFFFAOYSA-L
MDL number MFCD09953908
Appearance Brown or yellow solid

References for CNQX disodium salt

References are publications that support the biological activity of the product
  • The calpain inhibitor MDL-28170 and the AMPA/KA receptor antagonist CNQX inhibit neurofilament degradation and enhance neuronal survival in kainic acid-treated hippocampal slice cultures.

    Lopez-Picon FR et al (2006) Eur J Neurosci 23(10) : 2686-94.
  • 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) increases GABAA receptor-mediated spontaneous postsynaptic currents in the dentate granule cells of rat hippocampal slices.

    Hashimoto Y et al (2004) Neurosci Lett 358(1) : 33-6.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • 6,7-Dinitro-quinoxaline-2,3-dion and 6-nitro,7-cyano-quinoxaline-2,3-dion antagonise responses to NMDA in the rat spinal cord via an action at the strychnine-insensitive glycine receptor.

    Birch PJ et al (1988) Eur J Pharmacol 156(1) : 177-80.
Publications
These publications cite the use of CNQX disodium salt purchased from Hello Bio:
  • Downregulation of kainate receptors regulating GABAergic transmission in amygdala after early life stress is associated with anxiety-like behavior in rodents

    Englund et al (2021) Transl Psychiatry. 538. : 11(1)
    PubMedID: 34663781
  • Different glutamate sources and endogenous co-agonists activate extrasynaptic NMDA receptors on amacrine cells of the rod pathway microcircuit

    Beltran-Matas et al (2021) Eur J Neurosci. : 54(2)
    PubMedID: 34048091
  • Impaired cellular bioenergetics caused by GBA1 depletion sensitizes neurons to calcium overload

    Plotegher et al. (2020) Cell Death Differ. 27(5) : 1588-1603
    PubMedID: 31685979
  • Probing the ionotropic activity of glutamate GluD2 receptor in HEK cells with genetically-engineered photopharmacology

    Lemoine et al (2020) Elife 9: : e59026
    PubMedID: 33112237
  • Variance analysis as a tool to predict the mechanism underlying synaptic plasticity

    van Huijstee AN et al (2020) J Neurosci Methods 331 : 108526
    PubMedID: 31756397

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