Potent, selective and non-competitive NMDA receptor antagonist (Kd = 37.2 nM). Approx 10-fold more potent than (-)-MK 801 maleate. Prevents calcium ion influx and long term potentiation induction. Shows anticonvulsant and neuroprotective properties.
The NMDA receptor antagonist (+)-MK 801 is use-dependent and blocks NMDARs in their open conformation.
(+)-MK 801 from Hello Bio fully abolishes evoked NMDAR currents at 10 μM rapidly upon repeated stimulations (see Fig 1 above). At concentrations of 50 μM a more rapid receptor blockade was observed.
#Protocol 1: Evoked NMDA receptor currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
Neurons were held at +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
NMDA currents were continually stimulated and recorded in response to continual bath applications of (+)-MK 801 until NMDA currents were completely abolished.
All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.
Solubility & Handling
Soluble in water (25mM, gentle warming) and in DMSO (100mM)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.