Description Competitive NMDA receptor antagonist
DL-AP5: can't get a better deal than this! We love Hello Bio's DL-AP5!! This is one of the most heavily used inhibitors in our electrophysiology lab and with three rigs running daily we go through a LOT of it... Verified customer, University of Toronto Biological Data
DL-AP5 is a competitive NMDA receptor antagonist which binds at the glutamate site.
Impairs learning and fear conditioning.
DL-AP5 sodium salt also available.
DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 μM with full receptor antagonism achieved at 50 μM (see Fig 1 above). It was dissolved in water at 10 mM.
#Protocol 1: Evoked NMDA receptor currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg 2+ block.
NMDA currents were continually stimulated and recorded in response to continual bath applications of DL-AP5 until NMDA currents were completely abolished.
All NMDAR recordings were made in the presence of GABA A -R and AMPAR antagonists. Solubility & Handling
Storage instructions Room temperature
Solubility overview Soluble in water (10mM) or 0.1M NaOH (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. Chemical Data
Chemical name DL-2-Amino-5-phosphonopentanoic acid
Molecular Formula C 5H 12NO 5P
InChiKey VOROEQBFPPIACJ-UHFFFAOYSA-N References for DL-AP5
References are publications that support the biological activity of the product
Context-Dependent Modulation of Excitatory Synaptic Strength by Synaptically Released Zinc Kalappa and Tzounopoulos (2017) eNeuro 10.1523 : 0011-17
Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context. Laurent V et al (2009) Learn Mem 16(1) : 96-105. The basolateral amygdala is necessary for learning but not relearning extinction of context conditioned fear. Laurent V et al (2008) Learn Mem 15(5) : 304-14. Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of NMDA ionotropic glutamate receptor. Blaise MC et al (2005) J Mol Model 11(6) : 489-502.
These publications cite the use of DL-AP5 purchased from Hello Bio:
Endogenous TRPA1 and TRPV1 activity potentiates glutamatergic input to spinal lamina I neurons in inflammatory pain. Huang et al (2019) J Neurochem : doi: 10.1111/jnc.14677 [Epub ahe Circadian modulation of neurons and astrocytes controls synaptic plasticity in hippocampal area CA1 McCauley et al. (2019) bioRxiv preprint : 1-45 Functional and pharmacological properties of triheteromeric GluN1/2B/2D NMDA receptors Yi F et al (2019) J Physiol 597(22) : 5495-5514 Beyond the suprachiasmatic nucleus: circadian rhythmicity shapes astrocyte morphology and neuronal function in hippocampal area CA1 Scimemi et al (2019) bioRxiv : 10.1101/666073 Mechanisms of synaptic zinc plasticity at mouse dorsal cochlear nucleus glutamatergic synapses Tzounopoulos and Vogler (2018) bioRxiv doi : https://doi.org/10.1101/320671