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Product overview

Name DL-AP5
Alternative names DL-APV
Purity >99%
Customer comments

DL-AP5: can't get a better deal than this! We love Hello Bio's DL-AP5!! This is one of the most heavily used inhibitors in our electrophysiology lab and with three rigs running daily we go through a LOT of it... Verified customer, University of Toronto

Description Competitive NMDA receptor antagonist
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Figure 1. DL-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron

The NMDA receptor antagonist DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 µM with full receptor antagonism achieved at 50 µM. For assay protocol, see #Protocol 1 in Application Notes below
DL-AP5: Scientist Approved
DL-AP5 product vial image | Hello Bio

Biological Data

Biological description

DL-AP5 is a competitive NMDA receptor antagonist which binds at the glutamate site.

Impairs learning and fear conditioning.

Water soluble DL-AP5 sodium salt also available.

Application notes

DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 μM with full receptor antagonism achieved at 50 μM (see Fig 1 above). It was dissolved in water at 10 mM.


#Protocol 1: Evoked NMDA receptor currents

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
  • Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
  • NMDA currents were continually stimulated and recorded in response to continual bath applications of DL-AP5 until NMDA currents were completely abolished.
  • All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (10mM) or 0.1M NaOH (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Purity >99%
Chemical name DL-2-Amino-5-phosphonopentanoic acid
Molecular Weight 197.13
Chemical structure DL-AP5  [76326-31-3] Chemical Structure
Molecular Formula C5H12NO5P
CAS Number 76326-31-3
PubChem identifier 1216
Source Synthetic
InChi InChI=1S/C5H12NO5P/c6-4(5(7)8)2-1-3-12(9,10)11/h4H,1-3,6H2,(H,7,8)(H2,9,10,11)
MDL number MFCD00010515
Appearance White solid

References for DL-AP5

References are publications that support the biological activity of the product
  • Context-Dependent Modulation of Excitatory Synaptic Strength by Synaptically Released Zinc

    Kalappa and Tzounopoulos (2017) eNeuro 10.1523 : 0011-17
  • Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context.

    Laurent V et al (2009) Learn Mem 16(1) : 96-105.
  • The basolateral amygdala is necessary for learning but not relearning extinction of context conditioned fear.

    Laurent V et al (2008) Learn Mem 15(5) : 304-14.
  • Comparative analysis of different competitive antagonists interaction with NR2A and NR2B subunits of NMDA ionotropic glutamate receptor.

    Blaise MC et al (2005) J Mol Model 11(6) : 489-502.

4 Item(s)

These publications cite the use of DL-AP5 purchased from Hello Bio:
  • Long-Term Inactivation of Sodium Channels as a Mechanism of Adaptation in CA1 Pyramidal Neurons

    Canavier et al (2022) bioRxiv https://doi.org/10.1101/2021.10.26.465936 : doi
  • Theta-Burst Stimulation of Primary Afferents Drives Long-Term Potentiation in the Spinal Cord and Persistent Pain via α2σ-1-Bound NMDA Receptors

    Huang Y et al (2022) J Neurosci 42(3) : 513-527
    PubMedID: 34880118
  • Ketamine induces opposite changes in AMPA receptor calcium permeability in the ventral tegmental area and nucleus accumbens

    Skiteva O et al (2021) Transl Psychiatry 11(1) : 530
    PubMedID: 34650029
  • Nucleus Reuniens Afferents in Hippocampus Modulate CA1 Network Function via Monosynaptic Excitation and Polysynaptic Inhibition

    Goswamee P et al (2021) Front Cell Neurosci 15 : 660897
    PubMedID: 34712120
  • NMDA receptors are altered in the substantia nigra pars reticulata and their blockade ameliorates motor deficits in experimental parkinsonism

    Sitzia G et al (2020) Neuropharmacology 174 : 108136
    PubMedID: 32474027

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