DL-AP5: can't get a better deal than this! We love Hello Bio's DL-AP5!! This is one of the most heavily used inhibitors in our electrophysiology lab and with three rigs running daily we go through a LOT of it...Verified customer, University of Toronto
Figure 1. DL-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
The NMDA receptor antagonist DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 µM with full receptor antagonism achieved at 50 µM. For assay protocol, see #Protocol 1 in Application Notes below
DL-AP5: Scientist Approved
DL-AP5 product vial image | Hello Bio
Figure 1. DL-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
The NMDA receptor antagonist DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 µM with full receptor antagonism achieved at 50 µM. For assay protocol, see #Protocol 1 in Application Notes below
DL-AP5: Scientist Approved
DL-AP5 product vial image | Hello Bio
Biological Data
Biological description
DL-AP5 is a competitive NMDA receptor antagonist which binds at the glutamate site.
DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 μM with full receptor antagonism achieved at 50 μM (see Fig 1 above). It was dissolved in water at 10 mM.
#Protocol 1: Evoked NMDA receptor currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
NMDA currents were continually stimulated and recorded in response to continual bath applications of DL-AP5 until NMDA currents were completely abolished.
All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (10mM) or 0.1M NaOH (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References are publications that support the biological activity of the product
Context-Dependent Modulation of Excitatory Synaptic Strength by Synaptically Released Zinc
Kalappa and Tzounopoulos (2017) eNeuro 10.1523 : 0011-17
Infusion of the NMDA receptor antagonist, DL-APV, into the basolateral amygdala disrupts learning to fear a novel and a familiar context as well as relearning to fear an extinguished context.
![DL-AP5 [76326-31-3] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0251.png "DL-AP5 [76326-31-3]")
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