DL-AP5

DL-AP5 is a competitive NMDA receptor antagonist which binds at the glutamate site.

Impairs learning and fear conditioning.

Water soluble DL-AP5 sodium salt also available.

DL-AP5 from Hello Bio reduces the evoked NMDAR current at concentrations of 1 and 10 μM with full receptor antagonism achieved at 50 μM (see Fig 1 above). It was dissolved in water at 10 mM.

#Protocol 1: Evoked NMDA receptor currents

• Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
• NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
• Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg²⁺ block.
• NMDA currents were continually stimulated and recorded in response to continual bath applications of DL-AP5 until NMDA currents were completely abolished.
• All NMDAR recordings were made in the presence of GABA_A-R and AMPAR antagonists.