Product overview
Name | SR 95531 hydrobromide (Gabazine) |
Description | Selective, competitive GABAA receptor antagonist |
Alternative names | Gabazine | GBZ |
Purity | >98% |
Customer comments | We regularly use Hello Bio Gabazine (SR 95531) in the lab.We especially like the formulation where you only need to add 1ml of water to make a 10mM stock solution. Verified customer, The University of Newcastle I am satisfied with the quality, quick delivery and follow-up of your product. Verified customer, Shimane University We used our first aliquot of SR95531 (Gabazine) last week. The experiment was a critical one for us and the SR95531 worked exactly as expected – 100% block of a GABAergic IPSP (inhibitory postsynaptic potential). Verified customer, University of Michigan Good compound. This compound is routinely used in our lab to isolate AMPA and NMDA currents. So we are using it a lot every day. There are no complaints about it! Verified customer, Karolinska Institutet |
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Biological Data
Biological description | SR 95531 hydrobromide (Gabazine) is a selective and competitive GABAA receptor antagonist (Ki = 150 nM for displacement of [3H]-GABA from rat membranes).
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Application notes | Gabazine (SR 95531) is commonly used to reduce levels of inhibition by antagonising GABAA receptors. It is commonly used at concentrations between 10 – 200 μM. Gabazine (SR 95531) from Hello Bio blocks spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs (see Fig 1 above). It was effective at 1 μM and completely blocked GABAA receptors at 20 μM.
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
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Solubility & Handling
Storage instructions | Room temperature |
Solubility overview | Soluble in water (25mM) and in DMSO (100mM) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Chemical name | 6-Imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide |
Molecular Weight | 368.23 |
Chemical structure | |
Molecular Formula | C15H17N3O3.HBr |
CAS Number | 104104-50-9 |
PubChem identifier | 107895 |
SMILES | COC1=CC=C(C=C1)C2=NN(C(=N)C=C2)CCCC(=O)O.Br |
Source | Synthetic |
InChi | InChI=1S/C15H17N3O3.BrH/c1-21-12-6-4-11(5-7-12)13-8-9-14(16)18(17-13)10-2-3-15(19)20;/h4-9,16H,2-3,10H2,1H3,(H,19,20);1H |
InChiKey | GFZHNFOGCMEYTA-UHFFFAOYSA-N |
MDL number | MFCD00055135 |
Appearance | White solid |
References for SR 95531 hydrobromide (Gabazine)
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Sequential steps underlying neuronal plasticity induced by a transient exposure to gabazine.
Pegoraro S et al (2010) J Cell Physiol 222(3) : 713-28. -
The kinetics of inhibition of rat recombinant heteromeric alpha1beta glycine receptors by the low-affinity antagonist SR-95531.
Beato M et al (2007) J Physiol 580(Pt 1) : 171-9. -
Tonically activated GABAA receptors in hippocampal neurons are high-affinity, low-conductance sensors for extracellular GABA.
Yeung et al (2003) Mol Pharmacol 36(1) : 2-8. -
The differential antagonism by bicuculline and SR95531 of pentobarbitone-induced currents in cultured hippocampal neurons.
Uchida I et al (1996) Eur J Pharmacol 307(1) : 89-96. -
Biochemical characterization of the interaction of three pyridazinyl-GABA derivatives with the GABAA receptor site.
Heaulme M et al (1986) Brain Res 384(2) : 224-31.
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Examination of diurnal variation and sex differences in hippocampal neurophysiology and spatial memory
Goode et al (2022) BioRxiv https://www.biorxiv.org/content/10.1101/2022.03.12.484083v1 : . -
Synaptic inhibition in the lateral habenula shapes reward anticipation
Mameli et al (2021) bioRxiv . : https://doi.org/10.1101/2021.01. -
Probing the ionotropic activity of glutamate GluD2 receptor in HEK cells with genetically-engineered photopharmacology
Lemoine et al (2020) Elife 9: : e59026PubMedID: 33112237 -
Dopaminergic Transmission Rapidly and Persistently Enhances Excitability of D1 Receptor-Expressing Striatal Projection Neurons
Lahiri and Bevan (2020) Neuron 106(2) : 277-290PubMedID: 32075716 -
Neonatal ethanol exposure triggers apoptosis in the murine retrosplenial cortex: Role of inhibition of NMDA receptor-driven action potential firing
Bird CW et al (2020) Neuropharmacology 162 : 107837PubMedID: 31689422