We regularly use Hello Bio Gabazine (SR 95531) in the lab.We especially like the formulation where you only need to add 1ml of water to make a 10mM stock solution.Verified customer, The University of Newcastle
I am satisfied with the quality, quick delivery and follow-up of your product.
Verified customer, Shimane University
We used our first aliquot of SR95531 (Gabazine) last week. The experiment was a critical one for us and the SR95531 worked exactly as expected – 100% block of a GABAergic IPSP (inhibitory postsynaptic potential). Verified customer, University of Michigan
Good compound. This compound is routinely used in our lab to isolate AMPA and NMDA currents. So we are using it a lot every day. There are no complaints about it!Verified customer, Karolinska Institutet
Figure 1. Gabazine inhibition of evoked and spontaneous GABAA-R mediated IPSCs in mouse cortical neurons
Gabazine is commonly used to reduce levels of inhibition by antagonising GABAA receptors. It is commonly used at concentrations between 10 - 200 µM. Gabazine from Hello Bio blocks spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at 1 µM and completely blocked GABAA receptors at 20 µM. For assay protocol, see #Protocol 1 in Application Notes below
SR 95531 hydrobromide (Gabazine) product vial image | Hello Bio
Figure 1. Gabazine inhibition of evoked and spontaneous GABAA-R mediated IPSCs in mouse cortical neurons
Gabazine is commonly used to reduce levels of inhibition by antagonising GABAA receptors. It is commonly used at concentrations between 10 - 200 µM. Gabazine from Hello Bio blocks spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at 1 µM and completely blocked GABAA receptors at 20 µM. For assay protocol, see #Protocol 1 in Application Notes below
SR 95531 hydrobromide (Gabazine) product vial image | Hello Bio
Biological Data
Biological description
SR 95531 hydrobromide (Gabazine) is a selective and competitive GABAA receptor antagonist (Ki = 150 nM for displacement of [3H]-GABA from rat membranes).
SR 95531 (Gabazine) displaces GABA from the GABAAR agonist binding site to prevent receptor activation. It also acts as a negative allosteric inhibitor of channel opening to inhibit GABAA receptor activation by anaesthetic agents. It also displays low affinity glycine receptor inhibition.
SR 95531 (Gabazine) inhibits GABA-induced Cl- currents to reduce GABA-mediated synaptic inhibition.
SR 95531 additionally shows convulsive actions.
Application notes
Gabazine (SR 95531) is commonly used to reduce levels of inhibition by antagonising GABAA receptors. It is commonly used at concentrations between 10 – 200 μM.
Gabazine (SR 95531) from Hello Bio blocks spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs (see Fig 1 above). It was effective at 1 μM and completely blocked GABAA receptors at 20 μM.
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
A stimulating electrode was placed in layers II/III and IPSCs were evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable IPSC.
IPSCs were evoked at a range of neuron holding voltages to measure the reversal potential of the current to ensure it was GABAergic.
Neurons were held at 0mV and IPSCs continuously stimulated and recorded in response to 5 min applications of varying concentrations of Gabazine until complete receptor inhibition.
Spontaneous IPSCs were recorded before and after addition of Gabazine by holding the neuron at 0mV and recording for 10 sec.
All recordings for IPSCs were made in the presence of AMPAR antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (25mM) and in DMSO (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The positive allosteric modulator of NMDA receptors, GNE-9278, blocks the ethanol-induced decrease of excitability in developing retrosplenial cortex neurons from mice.
Bird CW et al (2023) Neuropsychopharmacology reports 43 : 77-84
![SR 95531 hydrobromide (Gabazine) [104104-50-9] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0901.png "SR 95531 hydrobromide (Gabazine) [104104-50-9]")
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