Kainic acid

(HB0355)
Technical documents: SDS CoA Datasheet

Product overview

Name Kainic acid
Description Prototypic, selective kainate receptor agonist. Potent excitant and neurotoxin.
Biological description

Kainic acid is the prototypic, selective kainate receptor (KAR) agonist. Analog of L-Glutamate.


Kainic acid is a potent excitant and neurotoxin (shows ~30-fold more neurotoxic potency than L-Glutamate).

It induces various changes in vivo including recurrent seizures, behavioural changes, oxidative stress, glial activation and selective neuronal death.


Kainic acid exerts its neuroexcitatory action by binding to glutamate receptors to cause Ca2+ influx and triggering subsequent excitotoxic neuronal death pathway cascades.

It is widely used to model various neurodegenerative diseases and epilepsy.

Purity >98%
Customer comments

We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus. Verified customer, CNRS, France

Exactly as ordered - we ordered 50 mg kainic acid and it arrived swiftly. The batch display same EC50 and efficacy as all other batches, so we assume it is pure and an intact batch of the compound. Verified customer, University of Copenhagen

Very happy - service from Hello Bio is always friendly and quick. Kainic acid (KA) works as expected on AMPA receptors in HEK cells. Much cheaper than alternative suppliers. Verified customer, UCL

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Biological Data

Application notes

The kainate receptor (KAR) agonist kainic acid induces neuronal oscillations in the gamma frequency band (35-100Hz). KAR activation leads to synchronous activity of excitatory and inhibitory network firing. Kainic acid is commonly used at concentrations of 400-600 nM to induce oscillations in acute brain slices. At a concentration of 550 nM, kainic acid from Hello Bio induces a strong gamma frequency neuronal oscillation (approx. 35Hz) within the CA3 region of the rat hippocampus (see Fig 1 above). Oscillations were abolished following kainic acid washout.

 

#Protocol 1: Oscillations assay

  • Acute rat hippocampal slices were placed in an interface chamber and held at 30°C with a glass recording electrode placed in the CA3 region of the hippocampus.
  • Control basal activity was recorded for 1 hr in the presence of aCSF before continuous perfusion of aCSF containing kainic acid at 550nM to induce oscillations.
  • Oscillation activity was recorded once oscillations had stabilised (~2-3 hours). 
  • Kainic acid was then washed out and replaced with control aCSF. 
  • Data were analysed to create a power spectrum of the oscillation.

 

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (25mM, gentle warming)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name (2S,3S,4S)-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid
Molecular Weight 213.23
Chemical structure Product image
Molecular Formula C10H15NO4
CAS Number 487-79-6
PubChem identifier 10255
SMILES CC(=C)[C@H]1CNC([C@H]1CC(=O)O)C(=O)O
Source Extracted from diginea simplex
InChi InChI=1S/C10H15NO4/c1-5(2)7-4-11-9(10(14)15)6(7)3-8(12)13/h6-7,9,11H,1,3-4H2,2H3,(H,12,13)(H,14,15)/t6-,7+,9-/m0/s1
InChiKey VLSMHEGGTFMBBZ-OOZYFLPDSA-N
MDL number MFCD00150833
Appearance White solid

References for Kainic acid

References are publications that support the biological activity of the product
  • Kainic acid-mediated excitotoxicity as a model for neurodegeneration.

    Wang Q et al (2005) Mol Neurobiol 31(1-3) : 3-16.
  • Distinct modulation of the endocannabinoid system upon kainic acid-induced in vivo seizures and in vitro epileptiform bursting.

    Fezza F et al (2014) Mol Cell Neurosci 62 : 1-9.
  • Identification and characterization of the ligand binding subunit of a kainic acid receptor using monoclonal antibodies and peptide mapping.

    Hampson DR et al (1989) J Biol Chem 264(22) : 13329-35.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
Publications
These publications cite the use of Kainic acid purchased from Hello Bio:
  • AMPA Receptor Inhibition by Medium-Chain Fatty Acids as a Mechanism for Seizure Control in the MCT Ketogenic Diet

    Augustin K (2017) Dissertation : 1:219
  • Regulation of AMPA receptors by type II-TARPs

    Lemos S (2017) Dissertation : 1:349
  • Auxiliary subunits keep AMPA receptors compact during activation and desensitization

    Baranovic and Plested (2018) bioRxiv https://doi.org : /10.1101/295105
  • AMPA receptor GluA2 subunit defects are a cause of neurodevelopmental disorders

    Salpietro V et al (2019) Nat Commun 10(1) : 3094
    PubMedID: 31300657
  • PTC-174, a positive allosteric modulator of NMDA receptors containing GluN2C or GluN2D subunits

    Yi F et al (2020) Neuropharmacology 25 : 107971
    PubMedID: 31987864

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