|Customer comments|| |
We tested the Kainic acid and we are very happy with it. Hello Bio Kainic acid was used at 25 mg/kg i.p. and successfully induced status epilepticus. Verified customer, CNRS, France
Exactly as ordered - we ordered 50 mg kainic acid and it arrived swiftly. The batch display same EC50 and efficacy as all other batches, so we assume it is pure and an intact batch of the compound. Verified customer, University of Copenhagen
Very happy - service from Hello Bio is always friendly and quick. Kainic acid (KA) works as expected on AMPA receptors in HEK cells. Much cheaper than alternative suppliers. Verified customer, UCL
|Description||Prototypic, selective kainate receptor agonist. Potent excitant and neurotoxin.|
Kainic acid induced gamma oscillations
Kainic acid: Scientist Approved
Kainic Acid product vial image | Hello Bio
|Biological description|| |
Kainic acid is the prototypic, selective kainate receptor (KAR) agonist. Analog of L-Glutamate.
It induces various changes in vivo including recurrent seizures, behavioural changes, oxidative stress, glial activation and selective neuronal death.
It is widely used to model various neurodegenerative diseases and epilepsy.
|Application notes|| |
The kainate receptor (KAR) agonist kainic acid induces neuronal oscillations in the gamma frequency band (35-100Hz). KAR activation leads to synchronous activity of excitatory and inhibitory network firing. Kainic acid is commonly used at concentrations of 400-600 nM to induce oscillations in acute brain slices. At a concentration of 550 nM, kainic acid from Hello Bio induces a strong gamma frequency neuronal oscillation (approx. 35Hz) within the CA3 region of the rat hippocampus (see Fig 1 above). Oscillations were abolished following kainic acid washout.
#Protocol 1: Oscillations assay
Solubility & Handling
|Storage instructions||Room temperature|
|Solubility overview||Soluble in water (25mM, gentle warming)|
|Important||This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.|
|Chemical name||(2S,3S,4S)-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid|
|Source||Extracted from diginea simplex|
References for Kainic acid
Distinct modulation of the endocannabinoid system upon kainic acid-induced in vivo seizures and in vitro epileptiform bursting.Fezza F et al (2014) Mol Cell Neurosci 62 : 1-9.
Kainic acid-mediated excitotoxicity as a model for neurodegeneration.Wang Q et al (2005) Mol Neurobiol 31(1-3) : 3-16.
Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.Alt et al (2004) Neuropharmacology 46(6) : 793-806
Identification and characterization of the ligand binding subunit of a kainic acid receptor using monoclonal antibodies and peptide mapping.Hampson DR et al (1989) J Biol Chem 264(22) : 13329-35.
Oscillatory Deficits in the Sub-Chronic PCP Rat Model for Schizophrenia Are Reversed by mGlu5 Receptor-Positive Allosteric Modulators VU0409551 and VU0360172.Brown J et al (2023) Cells 12PubMedID: 36980260
Dopamine D2 receptors in mossy cells reduce excitatory transmission and are essential for hippocampal function.Gulfo MC et al (2023) bioRxivPubMedID: 37205586
Human cortical interneurons optimized for grafting specifically integrate, abort seizures, and display prolonged efficacy without over-inhibition.Zhu Q et al (2022) NeuronPubMedID: 36626901
Activity-dependent LTP in the dentate gyrus promotes epileptic seizuresCastillo et al (2021) bioRxiv https://doi.org/10.1101/2021.06.30.450628 : doi
Ictal activation of oxygen-conserving reflexes as a mechanism for sudden death in epilepsyBiggs EN et al (2021) Epilepsia 62(3) : 752-764PubMedID: 33570173