NBQX disodium salt

(HB0443)
Technical documents: SDS CoA Datasheet

Product overview

Name NBQX disodium salt
Description Potent, selective, competitive AMPA receptor antagonist. Disodium salt.
Purity >99%
Customer comments

High quality and affordable! We use this compound routinely in the lab for neuronal recordings. Verified customer, The University of Montana

Worked just as it should, results indistinguishable from our previous product but at a significant cost reduction! Verified customer, The University of Toronto

Good quality and great price! Verified customer, The University of Newcastle

NBQX disodium salt produced by Hello Bio produced a very potent and "clean" block of synaptic AMPA currents, with no effect on other GABAA or NMDA receptors.Verified customer, The University of Edinburgh

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Images

NBQX disodium inhibition of AMPA receptor mediated EPSCs
NBQX disodium inhibition of glutamate stimulated calcium fluorescence
NBQX disodium: Scientist Approved
NBQX disodium salt product vial image | Hello Bio

Biological Data

Biological description

Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Water soluble, disodium salt. Shows neuroprotective, antinociceptive and anticonvulsive actions. NBQX also available.

Application notes

The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 μM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX disodium salt was also effective at reducing these currents at 1 μM. 

 

#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt
Molecular Weight 380.24
Chemical structure NBQX disodium salt  [479347-86-9] Chemical Structure
Molecular Formula C12H6N4Na2O6S
CAS Number 479347-86-9
PubChem identifier 3272523
SMILES [Na+].[Na+].NS(=O)(=O)c3cccc2c3c(cc1nc([O-])c([O-])nc12)[N+]([O-])=O
Source Synthetic
InChi InChI=1S/C12H8N4O6S.2Na/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6;;/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22);;/q;2*+1/p-2
InChiKey SVJKYIUJRJEABK-UHFFFAOYSA-L
MDL number MFCD12910445
Appearance Orange solid

References for NBQX disodium salt

References are publications that support the biological activity of the product
  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.
Publications
These publications cite the use of NBQX disodium salt purchased from Hello Bio:
  • Examination of diurnal variation and sex differences in hippocampal neurophysiology and spatial memory

    Goode et al (2022) BioRxiv https://www.biorxiv.org/content/10.1101/2022.03.12.484083v1 : .
  • Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors

    Beekhof et al (2021) Elife 2021 : online
    PubMedID: 33973524
  • Stress undermines reward-guided cognitive performance through synaptic depression in the lateral habenula

    Nuno-Perez et al (2021) Neuron. 109(6) : 947-956
    PubMedID: 33535028
  • Ultrastructural Correlates of Presynaptic Functional Heterogeneity in Hippocampal Synapse

    Maus et al. (2020) Cell Rep. 30(11) : 3632-3643
    PubMedID: 32187536
  • The entorhinal cortical alvear pathway differentially excites interneuron subtypes in hippocampal CA1

    Bell et al. (2020) bioRxiv preprint : 1-44

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