High quality and affordable! We use this compound routinely in the lab for neuronal recordings.Verified customer, The University of Montana
Worked just as it should, results indistinguishable from our previous product but at a significant cost reduction!Verified customer, The University of Toronto
Good quality and great price!Verified customer, The University of Newcastle
NBQX disodium salt produced by Hello Bio produced a very potent and "clean" block of synaptic AMPA currents, with no effect on other GABAA or NMDA receptors.Verified customer, The University of Edinburgh
Figure 1. NBQX disodium salt inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron
The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 µM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX disodium salt was also effective at reducing these currents at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. Percentage inhibiton of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
See Alt et al., 2004 for methodology and protocol
NBQX disodium: Scientist Approved
NBQX disodium salt product vial image | Hello Bio
Figure 1. NBQX disodium salt inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron
The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 µM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX disodium salt was also effective at reducing these currents at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. Percentage inhibiton of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
See Alt et al., 2004 for methodology and protocol
NBQX disodium: Scientist Approved
NBQX disodium salt product vial image | Hello Bio
Biological Data
Biological description
Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Water soluble, disodium salt. Blocks the induction of excitatory post synaptic currents. Shows neuroprotective, antinociceptive and anticonvulsive actions. NBQX also available.
Application notes
The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 μM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX disodium salt was also effective at reducing these currents at 1 μM.
#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
Recordings for EPSCs were made in the absence of GABAA-R antagonists.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in water (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
The positive allosteric modulator of NMDA receptors, GNE-9278, blocks the ethanol-induced decrease of excitability in developing retrosplenial cortex neurons from mice.
Bird CW et al (2023) Neuropsychopharmacology reports 43 : 77-84
![NBQX disodium salt [479347-86-9] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0443.png "NBQX disodium salt [479347-86-9]")
Understanding purity and quality - a guide for life scientists
