NBQX disodium salt

Technical documents: SDS CoA Datasheet

Product overview

Name NBQX disodium salt
Alternative names FG9202
Purity >99%
Customer comments

High quality and affordable! We use this compound routinely in the lab for neuronal recordings. Verified customer, The University of Montana

Worked just as it should, results indistinguishable from our previous product but at a significant cost reduction! Verified customer, The University of Toronto

Good quality and great price! Verified customer, The University of Newcastle

NBQX disodium salt produced by Hello Bio produced a very potent and "clean" block of synaptic AMPA currents, with no effect on other GABAA or NMDA receptors.Verified customer, The University of Edinburgh

Description Potent, selective, competitive AMPA receptor antagonist. Disodium salt.
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Figure 1. NBQX disodium salt inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron

The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 µM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX disodium salt was also effective at reducing these currents at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below

Figure 2. Percentage inhibiton of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2

See Alt et al., 2004 for methodology and protocol
NBQX disodium: Scientist Approved
NBQX disodium salt product vial image | Hello Bio

Biological Data

Biological description

Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Water soluble, disodium salt. Blocks the induction of excitatory post synaptic currents. Shows neuroprotective, antinociceptive and anticonvulsive actions. NBQX also available.

Application notes

The AMPA receptor antagonist NBQX disodium salt inhibits the actions of glutamate by acting at AMPARs and is commonly used at 10 μM. NBQX disodium salt from Hello Bio inhibits spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX disodium salt was also effective at reducing these currents at 1 μM. 


#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Purity >99%
Chemical name 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt
Molecular Weight 380.24
Chemical structure NBQX disodium salt  [479347-86-9] Chemical Structure
Molecular Formula C12H6N4Na2O6S
CAS Number 479347-86-9
PubChem identifier 3272523
SMILES [Na+].[Na+].NS(=O)(=O)c3cccc2c3c(cc1nc([O-])c([O-])nc12)[N+]([O-])=O
Source Synthetic
InChi InChI=1S/C12H8N4O6S.2Na/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6;;/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22);;/q;2*+1/p-2
MDL number MFCD12910445
Appearance Orange solid

References for NBQX disodium salt

References are publications that support the biological activity of the product
  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.

5 Item(s)

These publications cite the use of NBQX disodium salt purchased from Hello Bio:
  • Activity-dependent compartmentalization of dendritic mitochondria morphology through local regulation of fusion-fission balance in neurons in vivo.

    Virga DM et al (2024) Nature communications 15 : 2142
    PubMedID: 38459070
  • The positive allosteric modulator of NMDA receptors, GNE-9278, blocks the ethanol-induced decrease of excitability in developing retrosplenial cortex neurons from mice.

    Bird CW et al (2023) Neuropsychopharmacology reports 43 : 77-84
    PubMedID: 36524248
  • The selective dopamine D1 receptor agonist SKF81297 modulates NMDA receptor currents independently of D1 receptors

    Nesbit MO et al (2022) Neuropharmacology 207 : 108967
    PubMedID: 35077763
  • Examination of diurnal variation and sex differences in hippocampal neurophysiology and spatial memory

    Goode et al (2022) BioRxiv https://www.biorxiv.org/content/10.1101/2022.03.12.484083v1 : .
  • Gephyrin Interacts with the K-Cl Cotransporter KCC2 to Regulate Its Surface Expression and Function in Cortical Neurons

    Al Awabdh S et al (2022) J Neurosci 42(2) : 166-182
    PubMedID: 34810232

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