Figure 1. ß-actin expression in various tissue lysates and preparations visualised using HB8224
Figure 2. Concentration response of HB8224 in a rat brain cytosol preparation.
Figure 3. Histone H3 expression in various tissue lysates and preparations with ß-actin and GAPDH loading controls.
Figure 1. ß-actin expression in various tissue lysates and preparations visualised using HB8224
Figure 2. Concentration response of HB8224 in a rat brain cytosol preparation.
Figure 3. Histone H3 expression in various tissue lysates and preparations with ß-actin and GAPDH loading controls.
Product information
Immunogen
Recombinant fusion protein corresponding to residues 1-375 of human β-actin.
Purification
Immunogen affinity purification
Concentration
0.41 mg/mL
Formulation
PBS with 0.05% proclin300, 50% glycerol, pH7.3
Predicted species reactivity
Mouse, Rat, Human
Tested species reactivity
Mouse, Rat, Human
Tested applications
Applications
WB
Western blot optimal concentration
102ng/ml (1:4,000 dilution) as tested in a rat brain cytosol preparation
ICC optimal concentration
Not recommended for use in ICC
Positive control
β-actin is ubiquitously expressed at high levels in nearly all eukaryotic tissues and cells. It is also widely expressed in common cell lines.
Negative control
Due to the ubiquitous expression of β-actin, negative controls are challenging to create however since β-actin is a cytoskeletal protein, subcellular fractionation may be sufficient to provide a negative control. Due to its high expression, care should be taken to ensure that fractionation is complete without any cytoskeletal contamination.
Antibody to Histone H3 - Histone protein used as a loading control for western blotting. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Antibody to β-tubulin - cytoskeletal component widely used for imaging microtubules and as a loading control. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Western Blot Protocol (1 MB) 
