Figure 1. NBQX inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron
The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 µM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX was also effective at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below
NBQX: Scientist Approved
NBQX product vial image | Hello Bio
Figure 1. NBQX inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron
The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 µM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX was also effective at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below
NBQX: Scientist Approved
NBQX product vial image | Hello Bio
Biological Data
Biological description
Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Blocks the induction of excitatory post synaptic currents. Shows neuroprotective, antinociceptive and anticonvulsive actions. Water soluble, NBQX disodium salt also available.
Application notes
The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 μM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX was also effective at 1 μM. NBQX was dissolved in DMSO.
#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX until complete receptor inhibition.
Spontaneous EPSCs were recorded before and after addition of NBQX by holding the neuron at -70 mV and recording for 10 sec.
Recordings for EPSCs were made in the absence of GABAA-R antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in DMSO (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
These publications cite the use of NBQX purchased from Hello Bio:
Dysregulated clock gene expression and abnormal diurnal regulation of hippocampal inhibitory transmission and spatial memory in amyloid precursor protein transgenic mice
Fusilier AR et al (2021) Neurobiol Dis 158 : 105454
![NBQX [118876-58-7] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0442.png "NBQX [118876-58-7]")
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