NBQX

(HB0442)
Technical documents: SDS CoA Datasheet
2  Reviews
Top Reviews:
100% of 100
Add Your Review

Product overview

Name NBQX
Alternative names FG9202
Purity >98%
Description Potent, selective, competitive AMPA receptor antagonist
Write Your Own Review
You're reviewing:NBQX
Rate this item:

Images

Figure 1. NBQX inhibition of evoked and spontaneous glutamate mediated EPSCs in mouse cortical neuron

The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 µM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs). Complete AMPA receptor blockade was achieved at 10 µM and NBQX was also effective at 1 µM. For assay protocol, see #Protocol 1 in Application Notes below
NBQX: Scientist Approved
NBQX product vial image | Hello Bio

Biological Data

Biological description

Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Blocks the induction of excitatory post synaptic currents. Shows neuroprotective, antinociceptive and anticonvulsive actions. Water soluble, NBQX disodium salt also available.

Application notes

The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 μM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX was also effective at 1 μM. NBQX was dissolved in DMSO. 

 

#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of NBQX by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in DMSO (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

=
x
x
More Info

Dilution

x
=
x
More Info

Chemical Data

Purity >98%
Chemical name 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide
Molecular Weight 336.28
Chemical structure NBQX  [118876-58-7] Chemical Structure
Molecular Formula C12H8N4O6S
CAS Number 118876-58-7
PubChem identifier 3272524
SMILES C1=CC2=C3C(=CC(=C2C(=C1)S(=O)(=O)N)[N+](=O)[O-])NC(=O)C(=O)N3
Source Synthetic
InChi InChI=1S/C12H8N4O6S/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22)
InChiKey UQNAFPHGVPVTAL-UHFFFAOYSA-N
MDL number MFCD11046016
Appearance Yellow solid

References for NBQX

References are publications that support the biological activity of the product
  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.

5 Item(s)

Publications
These publications cite the use of NBQX purchased from Hello Bio:
  • Two opposing hippocampus to prefrontal cortex pathways for the control of approach and avoidance behaviour

    Sanchez-Bellot C et al (2022) Nat Commun 13(1) : 339
    PubMedID: 35039510
  • Pharmacological determination of the fractional block of Nav channels required to impair neuronal excitability and ex vivo seizures

    Parrish et al (2022) Biorxiv : https://doi.org/10.1101/2022.06.08.494063
  • Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors

    Beekhof et al (2021) Elife 2021 : online
    PubMedID: 33973524
  • Hippocampal ghrelin signalling informs the decision to eat

    MacAskill et al (2021) bioRxiv https://doi.org/10.1101/2021.11.05.467326 : doi
  • GluA4 facilitates cerebellar expansion coding and enables associative memory formation

    Kita K et al (2021) Elife 10
    PubMedID: 34219651

Items 1 to 5 of 9 total

Page