NBQX

(HB0442)
Technical documents: SDS CoA Datasheet

Product overview

Name NBQX
Description Potent, selective, competitive AMPA receptor antagonist
Alternative names NBQX
Purity >98%
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NBQX inhibition of AMPA receptor mediated EPSCs
NBQX: Scientist Approved

Biological Data

Biological description

Potent, selective and competitive AMPA receptor antagonist. Also kainate receptor antagonist. Shows neuroprotective, antinociceptive and anticonvulsive actions. Water soluble, NBQX disodium salt also available.

Application notes

The AMPA receptor antagonist NBQX inhibits the actions of glutamate acting at AMPARs and is commonly used at 10 μM. NBQX from Hello Bio reduces spontaneous and evoked excitatory post synaptic currents (EPSCs) (see Fig 1 above). Complete AMPA receptor blockade was achieved at 10 μM and NBQX was also effective at 1 μM. NBQX was dissolved in DMSO. 

 

#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of NBQX by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABAA-R antagonists.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in DMSO (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Chemical Data

Chemical name 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide
Molecular Weight 336.28
Chemical structure NBQX  [118876-58-7] Chemical Structure
Molecular Formula C12H8N4O6S
CAS Number 118876-58-7
PubChem identifier 3272524
SMILES C1=CC2=C3C(=CC(=C2C(=C1)S(=O)(=O)N)[N+](=O)[O-])NC(=O)C(=O)N3
Source Synthetic
InChi InChI=1S/C12H8N4O6S/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22)
InChiKey UQNAFPHGVPVTAL-UHFFFAOYSA-N
MDL number MFCD11046016
Appearance Yellow solid

References for NBQX

References are publications that support the biological activity of the product
  • It is AMPA receptor, not kainate receptor, that contributes to the NBQX-induced antinociception in the spinal cord of rats.

    Kong LL et al (2006) Brain Res 1100(1) : 73-7.
  • Pharmacological characterization of glutamatergic agonists and antagonists at recombinant human homomeric and heteromeric kainate receptors in vitro.

    Alt et al (2004) Neuropharmacology 46(6) : 793-806
  • Both MK801 and NBQX reduce the neuronal damage after impact-acceleration brain injury.

    Goda M et al (2002) J Neurotrauma 19(11) : 1445-56.
  • Antiepileptogenic and anticonvulsant effects of NBQX, a selective AMPA receptor antagonist, in the rat kindling model of epilepsy.

    Namba T et al (1994) Brain Res 638(1-2) : 36-44.
  • Competitive inhibition by NBQX of kainate/AMPA receptor currents and excitatory synaptic potentials: importance of 6-nitro substitution.

    Randle JC et al (1992) Eur J Pharmacol 215(2-3) : 237-44.
Publications
These publications cite the use of NBQX purchased from Hello Bio:
  • Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors

    Beekhof et al (2021) Elife 2021 : online
    PubMedID: 33973524
  • Kcnj6(GIRK2) trisomy is not sufficient for conferring the susceptibility to infantile spasms seen in the Ts65Dn mouse model of down syndrome.

    Joshi et al (2018) Epilepsy Res. 154 : 82-88.
    PubMedID: 29929098

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