I made the discovery that the NMDA receptor is the trigger for the induction of LTP using D-AP5 synthesized by Jeff Watkins, the discoverer of the NMDA receptor... I now obtain my D-AP5 from Hello Bio. I love their products and ethos and that is why I accepted a position on their Scientific Advisory Board.
Professor Graham Collingridge, winner of The Brain Prize, 2016
My lab used D-AP5 from Hello Bio and were very happy with it. It behaved exactly as expected! Professor Kei Cho, Chair of Neuroscience, University of Bristol, UK (Hello Bio Scientific Advisory Board Member)
My lab is very satisfied with your D-AP5 quality and price. Verified customer, European Brain Research Institute (EBRI)
I used to buy D-AP5 from another company, but Hello Bio is far more cost-effective and works great in our experiments.Verified customer, University of South Carolina
The D-AP5 works as expected, great price.Verified customer, UCSF
Figure 1. D-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 µM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 µM and reduces NMDA currents at lower concentrations of 1 and 10 µM. For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. D-AP5 inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 pyramidal neurones.
The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic events such as dendritic plateau potentials. D-AP5 from Hello Bio completely abolishes plateau potential formation at 50 µM. For protocol see #Protocol 2 in application notes below
D-AP5: Scientist Approved
High quality D-AP5 manufactured by Hello Bio
Figure 1. D-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 µM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 µM and reduces NMDA currents at lower concentrations of 1 and 10 µM. For assay protocol, see #Protocol 1 in Application Notes below
Figure 2. D-AP5 inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 pyramidal neurones.
The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic events such as dendritic plateau potentials. D-AP5 from Hello Bio completely abolishes plateau potential formation at 50 µM. For protocol see #Protocol 2 in application notes below
D-AP5: Scientist Approved
High quality D-AP5 manufactured by Hello Bio
Biological Data
Biological description
Widely used, selective and competitive NMDA receptor antagonist which binds at the glutamate site. It is the more active form of DL-AP5.
D-AP5 blocks induction of LTP (long term potentiation) in a reversible manner and is frequently used to inhibit NMDAR-mediated synaptic plasticity. Also impairs spatial learning.
Application notes
#Figure 1: D-AP5 inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 μM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 μM and reduces NMDA currents at lower concentrations of 1 and 10 μM (see Fig 1 above).
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#Protocol 1: Evoked NMDA receptor currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
NMDA currents were continually stimulated and recorded in response to continual bath applications of D-AP5 until NMDA currents were completely abolished.
All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.
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#Figure 2: D-AP5 inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 pyramidal neurones.
D-AP5 is commonly used to inhibit NMDA mediated synaptic events such as dendritic plateau potentials. Figure 2 shows that D-AP5 from Hello Bio completely abolishes plateau potential formation at 50µM (see Fig 2 above).
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#Protocol 2: Inhibition of NMDAR mediated dendritic plateau potentials in rat CA1 Pyramidal neurones
Pyramidal neurones from adult Wistar rats were patched in CA1 using a KMeSO4 internal solution with addition of 1mM QX-314 (HB1030) to prevent action potentials.
Cells were first held in Vclamp at -70mV for 10 minutes to wash out LTP before being transferred to Iclamp (again at -70mV) where they were stimulated sequentially every 15 seconds in the Schaffer collateral pathway.
Stimulation consisted of one single stimulation followed 400ms later by 5 stimulations at 100Hz.
Experiments took place in the presence of the GABAB antagonist GCP55845 (1µM, HB0960) and 50µM PTX.
Stimulation intensity was initially adjusted to evoke responses of approximately 1mV before stimulation was successively increased until robust plateau potentials were observed in all pathways.
Stimulation was then turned off and 50µM DAPV was washed on to the slice for 10 minutes before another stimulation response was conducted in the same cell.
Throughout the experiment input current was adjusted to maintain the cell at -70mV ± 0.5mV.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
You can solubilise D-AP5 in water, up to a concentration of 100 mM. Once D-AP5 is in suspension, if you have problems solubilising it, you can try:
Stirring – try rapidly stirring or vortexing in a whirlimixer
Heating – try warming it gently in a water bath
Sonicating – sonication may also be worth a try
Temperature is very important when solubilising biochemicals. For example if you’ve cooled or frozen your D-AP5 solution, it may have precipitated out of solution. So here’s an important rule – make sure you check that D-AP5 is fully re-dissolved before use.
Do I need to re-weigh the D-AP5 in my vial?
Yes - you should weigh out the quantity of D-AP5 that you require for your experiment as the amount of product in our vials isn’t weighed out accurately enough for direct addition of solution. There are some exceptions to this but if this is the case, it will be stated clearly on the datasheet.
References for D-AP5
References are publications that support the biological activity of the product
Age-dependent hippocampal network dysfunction in a mouse model of alpha-synucleinopathy
Tweedy et al (2018) Thessis : University of Newcastle
NMDA receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning.
Morris RG et al (2013) Eur J Neurosci 37(5) : 700-17.
Postsynaptic p47phox regulates long-term depression in the hippocampus
Cho et al (2018) Cell Discovery 4 : 44
Early life stress impairs postnatal oligodendrogenesis and adult behavior through activity-dependent mechanisms
Teissier et al (2018) bioRxiv https://doi.org/ : 10.1101/369660
DA neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Chuhma et al (2018) eLIFE 7:e39786 : DOI: 10.7554/eLife.39786
Activation of Serotonin 5-HT7 Receptors Modulates Hippocampal Synaptic Plasticity by Stimulation of Adenylate Cyclases and Rescues Learning and Behavior in a Mouse Model of Fragile X Syndrome.
Costa et al (2018) Front Mol Neurosci. 11:353 : doi: 10.3389/fnmol.2018.00353.
![D-AP5 APV [79055-68-8] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0225.png "D-AP5 APV [79055-68-8]")
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