Antibody to Calbindin - calcium binding protein used as a marker for an inhibitory interneuron subtype. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Calbindin is expressed in inhibitory interneurones in the brain with particularly high expression in the cerebellum and cortex alongside also being expressed in the kidney (collecting ducts and distal tubules) and retina.
Subcellular expression
Calbindin is primarily expressed in the cytosol of expressing cells with expression also having being reported in the nucleus.
Processing
Calbindin has the initiator methionine removed before forming a final conformation.
Post translational modifications
Calbindin is acetylated on alanine 2.
Homology (compared to human)
Mouse and rat calbindin have 98.5% identity with human calbindin. Mouse and rat calbindin show 99.2% homology (S60T and T232S).
Similar proteins
In a BLAST search Calretinin (58.5% identity, 29kDa) was the only protein identified with significant homology to Calbindin.
Storage & Handling
Storage instructions
-20°C
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Will my Calbindin antibody work against species that have not been listed on the datasheet?
A species not being listed doesn’t mean that the Calbindin antibody won’t work, just that we haven’t tested it. If you test one of our antibodies in a new species please let us know (positive or negative)!
What counterstains do you recommend for use in ICC and IHC with this Calbindin antibody?
What guarantee do you have that my Calbindin antibody will perform as expected?
We guarantee that your Calbindin antibody will work for the applications and species we list on the datasheet. If the antibody fails to perform as expected then we are happy to offer a 100% refund guarantee. For more details please see our guarantee policy.
What protocols are available for use with this Calbindin antibody?
We have made a comprehensive collection of protocols that we have used in our experiments to validate this Calbindin antibody.
What mounting media do you recommend to use with this antibody?
We recommend using one of our high performance mounting medias, supplied as either hardset or aqeous with a range of counterstains:
Antibody to Calbindin - calcium binding protein used as a marker for an inhibitory interneuron subtype. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Antibody to Calretinin - calcium binding protein used as a marker for an inhibitory interneuron subtype. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Antibody to Parvalbumin - calcium binding protein used as a marker for an inhibitory interneuron subtype. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Antibody to GAT1 - GABA reuptake transporter and marker for GABAergic interneurones. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Western Blot Protocol (1 MB) 
