Product overview
| Name | A 83-01 |
| Description | Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human hiPSC self renewal, 3D growth matrix component and promotes long-term organoid growth. Aids fibroblast to NSC/cardiomyocyte reprogramming. |
| Alternative names | A83 |
| Purity | >98% |
| Customer comments | : Good quality and very affordable price. My lab are very happy with this product from Hello Bio, we will definitely purchase this product again. Verified customer, UK |
Images
Biological Data
| Biological description | A 83-01 is a selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor (IC50 values are 12, 45 and 7.5 nM at ALK5, ALK4 and ALK7 respectively). It is more potent than SB431542 Reprogramming A 83-01 aids reprogramming of fibroblasts into neural stem cells and cardiomyocytes.
A 83-01 blocks phosphorylation of SMAD2/3 to inhibit TGF-β-induced epithelial-to-mesenchymal transition
A 83-01 helps to maintain homogeneity and long-term in vitro self-renewal of iPSCs
3D growth matrix component and also promotes long-term organoid growth. |
| Application notes | Figures 1 - 3 show liver hepatocellular carcinoma tumouroids cultured with A 83-01 and forskolin |
Solubility & Handling
| Storage instructions | -20°C (protect from light) |
| Solubility overview | Soluble in DMSO (50mM) |
| Handling |
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| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Chemical name | 3-(6-Methyl-2-pyridinyl)-N-phenyl-4 -(4-quinolinyl)-1H-pyrazole-1-carbothioamide |
| Molecular Weight | 421.52 |
| Chemical structure | ![A 83-01 | [909910-43-6] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb3218_6.png "A 83-01 | [909910-43-6]") |
| Molecular Formula | C25H19N5S |
| CAS Number | 909910-43-6 |
| PubChem identifier | 16218924 |
| SMILES | CC1=CC=CC(=N1)C2=NN(C=C2C3=CC=NC4=CC=CC=C34)C(=S)NC5=CC=CC=C5 |
| InChi | InChI=1S/C25H19N5S/c1-17-8-7-13-23(27-17)24-21(19-14-15-26-22-12-6-5-11-20(19)22)16-30(29-24)25(31)28-18-9-3-2-4-10-18/h2-16H,1H3,(H,28,31) |
| InChiKey | HIJMSZGHKQPPJS-UHFFFAOYSA-N |
| Appearance | Pale yellow solid |
References for A 83-01
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Chemical compound-based direct reprogramming for future clinical applications.
Takeda et al (2018) Biosci Rep 38(3) : pii: BSR20171650 -
Brief report: combined chemical treatment enables Oct4-induced reprogramming from mouse embryonic fibroblasts.
Yuan et al (2011) 2011 29(3) : 549-53 -
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.
Li et al (2009) Cell Stem Cell 4(1) : 16-9 -
The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta.
Tojo et al (2005) Cancer Sci 96(11) : 791-800
Technical guides
Understanding purity and quality - a guide for life scientists
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