Good quality and very affordable price. My lab are very happy with this product from Hello Bio, we will definitely purchase this product again.Verified customer, UK
Description
Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human hiPSC self renewal, 3D growth matrix component and promotes long-term organoid growth. Aids fibroblast to NSC/cardiomyocyte reprogramming.
Figure. 1 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Figure. 2 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Figure. 3 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Figure. 1 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Figure. 2 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Figure. 3 Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Liver hepatocellular carcinoma tumouroids cultured with A83-01 (HB3218) and Forskolin (HB1348)
Biological Data
Biological description
A 83-01 is a selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor (IC50 values are 12, 45 and 7.5 nM at ALK5, ALK4 and ALK7 respectively). It is more potent than SB431542
Reprogramming
A 83-01 aids reprogramming of fibroblasts into neural stem cells and cardiomyocytes.
Differentiation
A 83-01 blocks phosphorylation of SMAD2/3 to inhibit TGF-β-induced epithelial-to-mesenchymal transition
Maintenance / self-renewal
A 83-01 helps to maintain homogeneity and long-term in vitro self-renewal of iPSCs
Organoids
3D growth matrix component and also promotes long-term organoid growth.
Application notes
Figures 1 - 3 show liver hepatocellular carcinoma tumouroids cultured with A 83-01 and forskolin
Solubility & Handling
Storage instructions
-20°C (protect from light)
Solubility overview
Soluble in DMSO (50mM)
Handling
This compound is light sensitive; we therefore recommend protecting the solid material and solutions from exposure to light.
It has been reported that A 83-01 can decompose to A 77-01 in solution. We therefore recommend that you make up solutions and use immediately. Do not store solutions.
If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human hiPSC self renewal, 3D growth matrix component and promotes long-term organoid growth. Aids fibroblast to NSC/cardiomyocyte reprogramming.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
![A 83-01 | [909910-43-6] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb3218_6.png "A 83-01 | [909910-43-6]")
Understanding purity and quality - a guide for life scientists
