A 83-01

(HB3218)
Technical documents: SDS CoA Datasheet

Product overview

Name A 83-01
Description Selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor. Maintains human hiPSC self renewal, 3D growth matrix component and promotes long-term organoid growth. Aids fibroblast to NSC/cardiomyocyte reprogramming.
Biological description

A 83-01 is a selective TGF-βRI (ALK5), ALK4 and ALK7 inhibitor (IC50 values are 12, 45 and 7.5 nM at ALK5, ALK4 and ALK7 respectively). It is more potent than SB431542

Reprogramming

A 83-01 aids reprogramming of fibroblasts into neural stem cells and cardiomyocytes.


Differentiation

A 83-01 blocks phosphorylation of SMAD2/3 to inhibit TGF-β-induced epithelial-to-mesenchymal transition


Maintenance / self-renewal

A 83-01 helps to maintain homogeneity and long-term in vitro self-renewal of iPSCs


Organoids

3D growth matrix component and also promotes long-term organoid growth.

Alternative names A83
Purity >98%
Customer comments

: Good quality and very affordable price. My lab are very happy with this product from Hello Bio, we will definitely purchase this product again. Verified customer, UK

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Images

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Biological Data

Application notes

Figures 1 - 3 show liver hepatocellular carcinoma tumouroids cultured with A 83-01 and forskolin

Solubility & Handling

Storage instructions -20°C (protect from light)
Solubility overview Soluble in DMSO (50mM)
Handling

  • This compound is light sensitive; we therefore recommend protecting the solid material and solutions from exposure to light.
  • It has been reported that A 83-01 can decompose to A 77-01 in solution. We therefore recommend that you make up solutions and use immediately. Do not store solutions.
  • If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month.

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name 3-(6-Methyl-2-pyridinyl)-N-phenyl-4 -(4-quinolinyl)-1H-pyrazole-1-carbothioamide
Molecular Weight 421.52
Chemical structure Product image
Molecular Formula C25H19N5S
CAS Number 909910-43-6
PubChem identifier 16218924
SMILES CC1=CC=CC(=N1)C2=NN(C=C2C3=CC=NC4=CC=CC=C34)C(=S)NC5=CC=CC=C5
InChi InChI=1S/C25H19N5S/c1-17-8-7-13-23(27-17)24-21(19-14-15-26-22-12-6-5-11-20(19)22)16-30(29-24)25(31)28-18-9-3-2-4-10-18/h2-16H,1H3,(H,28,31)
InChiKey HIJMSZGHKQPPJS-UHFFFAOYSA-N
Appearance Pale yellow solid

References for A 83-01

References are publications that support the biological activity of the product
  • The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta.

    Tojo et al (2005) Cancer Sci 96(11) : 791-800
  • Brief report: combined chemical treatment enables Oct4-induced reprogramming from mouse embryonic fibroblasts.

    Yuan et al (2011) 2011 29(3) : 549-53
  • Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.

    Li et al (2009) Cell Stem Cell 4(1) : 16-9
  • Chemical compound-based direct reprogramming for future clinical applications.

    Takeda et al (2018) Biosci Rep 38(3) : pii: BSR20171650