The AMPA receptor agonist (S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).
#Protocol 1: (S)-AMPA protocol
Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
AMPA currents were evoked via applying (S)-AMPA directly to the recording chamber during continuous perfusion.
To test the selectivity of (S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
Under these conditions (S)-AMPA failed to induce a depolarising current.
Solubility & Handling
Soluble in water (100mM)
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