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AMPAR mediated whole cell current in response to S-AMPA
(S)-AMPA: Scientist Approved
(S)-AMPA product vial image | Hello Bio

Biological Data

Biological description

(S)-AMPA is an AMPA receptor agonist and the active enantiomer of AMPA. It is a neurotoxin in the immature rat brain.

(R,S)-AMPA is also available.

Application notes

The AMPA receptor agonist (S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).


#Protocol 1: (S)-AMPA protocol

  • Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
  • Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
  • AMPA currents were evoked via applying (S)-AMPA directly to the recording chamber during continuous perfusion.
  • To test the selectivity of (S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
  • Under these conditions (S)-AMPA failed to induce a depolarising current.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name (S)-α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
Molecular Weight 186.17
Chemical structure (S)-AMPA  [83643-88-3] Chemical Structure
Molecular Formula C7H10N2O4
CAS Number 83643-88-3
PubChem identifier 158397
SMILES CC1=C(C(=O)NO1)C[C@@H](C(=O)O)N
Source Synthetic
InChi InChI=1S/C7H10N2O4/c1-3-4(6(10)9-13-3)2-5(8)7(11)12/h5H,2,8H2,1H3,(H,9,10)(H,11,12)/t5-/m0/s1
InChiKey UUDAMDVQRQNNHZ-YFKPBYRVSA-N
MDL number MFCD00672630
Appearance White solid

References for (S)-AMPA

References are publications that support the biological activity of the product
  • The selective ionotropic-type quisqualate receptor agonist AMPA is a potent neurotoxin in immature rat brain.

    McDonald JW et al (1990) Brain Res 526(1) : 165-8.
  • Ibotenic acid analogues. Synthesis, molecular flexibility, and in vitro activity of agonists and antagonists at central glutamic acid receptors.

    Lauridsen J et al (1985) J Med Chem 28(5) : 668-72.
  • Enzymic resolution and binding to rat brain membranes of the glutamic acid agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid.

    Hansen JJ et al (1983) J Med Chem 26(6) : 901-3.
Publications
These publications cite the use of (S)-AMPA purchased from Hello Bio:
  • Synaptic dysregulation and hyperexcitability induced by intracellular amyloid beta oligomers

    Fernandez-Perez EJ et al (2021) Aging Cell 20(9) : e13455
    PubMedID: 34409748
  • PKD promotes activity-dependent AMPA receptor endocytosis in hippocampal neurons

    Morales et al. (2020) bioRxiv preprint : 1-27

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