Figure 1. DNQX inhibition of evoked and spontaneous EPSCs in mouse coritical neurons
DNQX antagonised the actions of glutamate at AMPA receptos. It is commonly used to reduce excitatory post synaptic currents (EPSC) and is commonly used at 10 µM. DNQX from Hello Bio completely blocks both spontaneous and evoked EPSCs at 10 µM, with concentrations of 1 µM also effective. For assay protocol, see #Protocol 1 in Application Notes below
Percentage Inhibition of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
DNQX: Scientist Approved
DNQX product vial image | Hello Bio
Figure 1. DNQX inhibition of evoked and spontaneous EPSCs in mouse coritical neurons
DNQX antagonised the actions of glutamate at AMPA receptos. It is commonly used to reduce excitatory post synaptic currents (EPSC) and is commonly used at 10 µM. DNQX from Hello Bio completely blocks both spontaneous and evoked EPSCs at 10 µM, with concentrations of 1 µM also effective. For assay protocol, see #Protocol 1 in Application Notes below
Percentage Inhibition of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
DNQX: Scientist Approved
DNQX product vial image | Hello Bio
Biological Data
Biological description
DNQX is a selective and competitive AMPA and kainate receptor antagonist. DNQX allso acts as partial AMPA agonist in the presence of γ2 transmembrane AMPA receptor regulatory proteins (TARP) subunit.
DNQX is also a neuroleptic agent that displays pro-oxidant activity.
DNQX antagonizes the actions of glutamate at AMPA receptors. It is commonly used to reduce excitatory post synaptic currents (EPSC) and is commonly used at 10 μM. DNQX disodium salt from Hello Bio completely blocks both spontaneous and evoked EPSCs at 10 μM, with concentrations of 1 μM also effective (see Fig 1 above).
#Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
Recordings for EPSCs were made in the absence of GABAA-R antagonists.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in DMSO (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![DNQX [2379-57-9] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0261.png "DNQX [2379-57-9]")
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