Figure 1. AMPAR mediated whole-cell current in rat CA1 pyramidal in response to application of (RS)-AMPA
The AMPA receptor agonist (RS)–AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (RS)–AMPA from Hello Bio induced a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20 µM). For assay protocol, see #Protocol 1 in Application Notes below.
(R,S)-AMPA: Scientist Approved
(R,S)-AMPA product vial image | Hello Bio
Figure 1. AMPAR mediated whole-cell current in rat CA1 pyramidal in response to application of (RS)-AMPA
The AMPA receptor agonist (RS)–AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (RS)–AMPA from Hello Bio induced a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20 µM). For assay protocol, see #Protocol 1 in Application Notes below.
(R,S)-AMPA: Scientist Approved
(R,S)-AMPA product vial image | Hello Bio
Biological Data
Biological description
Prototypic AMPA receptor agonist (EC50 = 11 μM). (S)-AMPA is the active enantiomer form.
Application notes
The AMPA receptor agonist (R,S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (R,S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).
#Protocol 1: (R,S)-AMPA protocol
Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
AMPA currents were evoked via applying (R,S)-AMPA directly to the recording chamber during continuous perfusion.
To test the selectivity of (R,S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
Under these conditions (R,S)-AMPA failed to induce a depolarising current.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (10mM, gentle warming)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![(R,S)-AMPA [77521-29-0] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0030.png "(R,S)-AMPA [77521-29-0]")
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