Prototypic AMPA receptor agonist (EC50 = 11 μM). (S)-AMPA is the active enantiomer form.
The AMPA receptor agonist (R,S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (R,S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).
#Protocol 1: (R,S)-AMPA protocol
Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
AMPA currents were evoked via applying (R,S)-AMPA directly to the recording chamber during continuous perfusion.
To test the selectivity of (R,S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
Under these conditions (R,S)-AMPA failed to induce a depolarising current.
Solubility & Handling
Soluble in water (10mM, gentle warming)
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.