Cyclothiazide

Cyclothiazide (CTZ) is an AMPA receptor positive allosteric modulator (PAM) which produces a fast inhibition of AMPAR desensitization and a slow potentiation of the AMPA current.

Cyclothiazide also inhibits GABA_A mediated currents and shows diuretic and convulsive actions.

Cyclothiazide is active in vivo.

The AMPAR positive allosteric modulator Cyclothiazide increases the open time of the AMPAR by inhibiting AMPAR desensitization. It is often used at a concentration of 100 μM. Cyclothiazide from Hello Bio potentiates AMPAR mediated evoked EPSCs in cortical neurons at 50 μM and above (see Fig 1 above)

#Protocol 1: Evoked and spontaneous evoked excitatory post synaptic currents (EPSCs)

• Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
• EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC. 
• Neurons were held at -70 mV (the reversal potential of GABA currents).
• EPSCs were then continuously stimulated and recorded in response to 5 min applications of 50 μM and then 100 μM Cyclothiazide.