Product overview
| Name | RuBi-Glutamate |
| Description | RuBi - caged glutamate compound. Water soluble. |
| Biological description | Ruthenium-bipyridine-trimethylphosphine (RuBi) - caged glutamate. Water soluble.
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| Alternative names | RuBi-Glu, RuBIglutamate |
| Customer comments | Hello Bio produce very high quality Rubi-Glutamate, which is capable of evoking single spine synaptic currents following 2-photon activation. The spatial and temporal dynamics of the uncaging are suitable for fast photolysis, mimicking accurately endogenous glutamate release. Verified customer, The University of Edinburgh |
Images
Biological Data
| Application notes | Uncaging of Rubi-Glutamate using a 0.5 µm spot of 780 nm light for 2 ms (blue line), 1 µm adjacent to dendritic spines (average of 8 spine shown) in a CA1 pyramidal cell from a 28 day old rat. Traces were acquired using a cesium gluconate based intracellular solution in voltage-clamp configuration, in the presence of bath applied 300 µM Rubi-Glutamate and 50 µM picrotoxin (also Hello Bio), producing small AMPA EPSCs at -70 mV (top) and large NMDA EPSCs at +40 mV (bottom). |
Solubility & Handling
| Storage instructions | -20°C |
| Solubility overview | Soluble in water (20mM) |
| Handling | This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the material in the dark and protecting from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Chemical name | (bis(2,2'-Bipyridine-N,N')trimethylphosphine)-(S)-1-aminopropane-1,3-dicarboxylic acid ruthenium(2+) complex sodium hexafluorophosphate salt |
| Molecular Weight | 970.54 |
| Chemical structure |  |
| Molecular Formula | C28H32N5O4PRu.2NaPF6 |
| CAS Number | 2417096-44-5 |
| PubChem identifier | 90488860 |
| SMILES | CP(C)C.C1=CC=NC(=C1)C2=CC=CC=N2.C1=CC=NC(=C1)C2=CC=CC=N2.C(CC(=O)[O-])C(C(=O)[O-])N.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.[Na+].[Na+].[Ru+2] |
| Source | Synthetic |
| InChiKey | SQQVSHWHIPVYSZ-UHFFFAOYSA-L |
| Appearance | Orange solid |
References for RuBi-Glutamate
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Dense, unspecific connectivity of neocortical parvalbumin-positive interneurons: a canonical microcircuit for inhibition?
Packer AM et al (2011) J Neurosci 31(37) : 13260-71. -
Dense inhibitory connectivity in neocortex.
Fino and Yuste (2011) Neuron. 69(6) : 1188-203. -
A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes.
Salierno M et al (2010) J Inorg Biochem 104(4) : 418-22. -
RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines.
Fino E et al (2009) Front Neural Circuits 3 : 2
Technical guides
Understanding purity and quality - a guide for life scientists
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