Product overview
Name | Picrotoxin |
Description | Non-competitive GABAA receptor antagonist |
Alternative names | PTX |
Purity | >98% |
Customer comments | Getting on well with DHPG & picrotoxin – they do what they’re supposed to! Professor Bruno Frenguelli, University of Warwick, UK Reasonable price and good working! Verified customer, Seoul National University |
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Biological Data
Biological description | Non-competitive GABAA receptor antagonist. Also a glycine receptor inhibitor (IC50 = 2.7 µM). Acts as a convulsant and CNS stimulant. Active in vivo. |
Application notes | The GABAA receptor antagonist Picrotoxin is commonly used to reduce the levels inhibition by blocking the actions of the neurotransmitter GABA. Picrotoxin from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at concentrations of 10μM, with complete receptor blockade at 100μM. For assay protocol, see #Protocol 1 in Application Notes below
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
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Solubility & Handling
Storage instructions | Room temperature |
Solubility overview | Soluble in DMSO (100mM) and in ethanol (50mM, gentle warming) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Molecular Weight | 602.59 |
Chemical structure | |
Molecular Formula | C30H34O13 |
CAS Number | 124-87-8 |
PubChem identifier | 518601 |
SMILES | CC(=C)C1C2C3C4(C(C1C(=O)O2)(CC5C4(O5)C(=O)O3)O)C.CC12C3C4C(C(C1(CC5C2(O5)C(=O)O3)O)C(=O)O4)C(C)(C)O |
InChi | InChI=1S/C15H18O7.C15H16O6/c1-12(2,18)6-7-10(16)20-8(6)9-13(3)14(7,19)4-5-15(13,22-5)11(17)21-9;1-5(2)7-8-11(16)19-9(7)10-13(3)14(8,18)4-6-15(13,21-6)12(17)20-10/h5-9,18-19H,4H2,1-3H3;6-10,18H,1,4H2,2-3H3 |
InChiKey | VJKUPQSHOVKBCO-UHFFFAOYSA-N |
MDL number | MFCD00074824 |
References for Picrotoxin
References are publications that support the biological activity of the product
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Picrotoxin-like channel blockers of GABAA receptors.
Olsen RW (2006) Proc Natl Acad Sci U S A 103(16) : 6081-2. -
Mechanisms for picrotoxin block of alpha2 homomeric glycine receptors.
Wang DS et al (2006) J Biol Chem 281(7) : 3841-55. -
Picrotoxin blockade of invertebrate glutamate-gated chloride channels: subunit dependence and evidence for binding within the pore.
Etter A et al (1999) J Neurochem 72(1) : 318-26.
Publications
These publications cite the use of Picrotoxin purchased from Hello Bio:
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Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors
Beekhof et al (2021) Elife 2021 : onlinePubMedID: 33973524 -
Downregulation of kainate receptors regulating GABAergic transmission in amygdala after early life stress is associated with anxiety-like behavior in rodents
Englund et al (2021) Transl Psychiatry. 538. : 11(1)PubMedID: 34663781 -
Multiple roles of GluN2D-containing NMDA receptors in short-term potentiation and long-term potentiation in mouse hippocampal slices
Eapen et al (2021) Neuropharmacology . 108833 : 201PubMedID: 34637787 -
Synaptic inhibition in the lateral habenula shapes reward anticipation
Mameli et al (2021) bioRxiv . : https://doi.org/10.1101/2021.01. -
Stress undermines reward-guided cognitive performance through synaptic depression in the lateral habenula
Nuno-Perez et al (2021) Neuron. 109(6) : 947-956PubMedID: 33535028