Product overview
Name | Picrotoxin |
Description | Non-competitive GABAA receptor antagonist |
Alternative names | PTX |
Purity | >98% |
Customer comments | Getting on well with DHPG & picrotoxin – they do what they’re supposed to! Professor Bruno Frenguelli, University of Warwick, UK Reasonable price and good working! Verified customer, Seoul National University |
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Biological Data
Biological description | Non-competitive GABAA receptor antagonist. Also a glycine receptor inhibitor (IC50 = 2.7 µM). Acts as a convulsant and CNS stimulant. Active in vivo. |
Application notes | The GABAA receptor antagonist Picrotoxin is commonly used to reduce the levels inhibition by blocking the actions of the neurotransmitter GABA. Picrotoxin from Hello Bio reduces both spontaneous inhibitory post synaptic currents (IPSC) and evoked IPSCs. It was effective at concentrations of 10μM, with complete receptor blockade at 100μM. For assay protocol, see #Protocol 1 in Application Notes below
#Protocol 1: Evoked and spontaneous inhibitory post synaptic currents (IPSCs)
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Solubility & Handling
Storage instructions | Room temperature |
Solubility overview | Soluble in DMSO (100mM) and in ethanol (50mM, gentle warming) |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Purity | >98% |
Molecular Weight | 602.59 |
Chemical structure | |
Molecular Formula | C30H34O13 |
CAS Number | 124-87-8 |
PubChem identifier | 518601 |
SMILES | CC(=C)C1C2C3C4(C(C1C(=O)O2)(CC5C4(O5)C(=O)O3)O)C.CC12C3C4C(C(C1(CC5C2(O5)C(=O)O3)O)C(=O)O4)C(C)(C)O |
InChi | InChI=1S/C15H18O7.C15H16O6/c1-12(2,18)6-7-10(16)20-8(6)9-13(3)14(7,19)4-5-15(13,22-5)11(17)21-9;1-5(2)7-8-11(16)19-9(7)10-13(3)14(8,18)4-6-15(13,21-6)12(17)20-10/h5-9,18-19H,4H2,1-3H3;6-10,18H,1,4H2,2-3H3 |
InChiKey | VJKUPQSHOVKBCO-UHFFFAOYSA-N |
MDL number | MFCD00074824 |
References for Picrotoxin
References are publications that support the biological activity of the product
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Picrotoxin-like channel blockers of GABAA receptors.
Olsen RW (2006) Proc Natl Acad Sci U S A 103(16) : 6081-2. -
Mechanisms for picrotoxin block of alpha2 homomeric glycine receptors.
Wang DS et al (2006) J Biol Chem 281(7) : 3841-55. -
Picrotoxin blockade of invertebrate glutamate-gated chloride channels: subunit dependence and evidence for binding within the pore.
Etter A et al (1999) J Neurochem 72(1) : 318-26.
Publications
These publications cite the use of Picrotoxin purchased from Hello Bio:
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Edinger-Westphal peptidergic neurons enable maternal preparatory nesting
Topilko T et al (2022) NeuronPubMedID: 35123655 -
Transient developmental increase in cortical projections to amygdala GABAergic neurons contribute to circuit dysfunction following early life stress
Lauri et al (2022) Biorxiv : https://doi.org/10.1101/2022.04.21.489031 -
Sex differences in a-adrenergic receptor function contribute to impaired hypothalamic metaplasticity following chronic intermittent ethanol exposure
Munier JJ et al (2022) Alcohol Clin Exp ResPubMedID: 35791038 -
Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors
Beekhof et al (2021) Elife 2021 : onlinePubMedID: 33973524 -
Downregulation of kainate receptors regulating GABAergic transmission in amygdala after early life stress is associated with anxiety-like behavior in rodents
Englund et al (2021) Transl Psychiatry. 538. : 11(1)PubMedID: 34663781