Figure 1. NMDA mediated whole-cell current in mouse cortical neuron
The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 µM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 µM with prominent currents at 50 µM. The actions of NMDA were fully blocked by D-AP5 (NMDAR antagonist) at 100 µM. For assay protocol, see #Protocol 1 in Application Notes below.
NMDA: Scientist Approved
NMDA product vial image | Hello Bio
Figure 1. NMDA mediated whole-cell current in mouse cortical neuron
The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 µM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 µM with prominent currents at 50 µM. The actions of NMDA were fully blocked by D-AP5 (NMDAR antagonist) at 100 µM. For assay protocol, see #Protocol 1 in Application Notes below.
NMDA: Scientist Approved
NMDA product vial image | Hello Bio
Biological Data
Biological description
Prototypic NMDA receptor agonist which mimics the action of glutamate. Potent excitant.
Application notes
The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 μM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 μM with prominent currents at 50 μM. The actions of NDMA were fully blocked by D-AP5 (NMDAR antagonist) at 100 μM (see Fig 1 above).
#Protocol 1: NMDA mediated whole-cell currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
Neurons were held at -70 mV and continuously perfused with aCSF in the presence of AMPA and GABA receptor antagonists CNQX (10 μM) and Bicuculline (100 μM) respectively and Tetrodotoxin (1 μM) to reduce network activity.
NMDA currents were evoked by applying NMDA directly to the recording chamber during continuous perfusion. • To test the selectivity of NMDA to NMDA receptors the experiment was repeated within the same neuron in the presence of the NMDA receptor antagonist D-AP5 (100 μM). Under these conditions NMDA failed to induce a depolarising current.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in water (100mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
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