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NMDA induction of inward depolarising whole-cell currents
NMDA: Scientist Approved
NMDA product vial image | Hello Bio

Biological Data

Biological description Prototypic NMDA receptor agonist which mimics the action of glutamate. Potent excitant.
Application notes

The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 μM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 μM with prominent currents at 50 μM. The actions of NDMA were fully blocked by D-AP5 (NMDAR antagonist) at 100 μM (see Fig 1 above).

 

#Protocol 1: NMDA mediated whole-cell currents

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • Neurons were held at -70 mV and continuously perfused with aCSF in the presence of AMPA and GABA receptor antagonists CNQX (10 μM) and Bicuculline (100 μM) respectively and Tetrodotoxin (1 μM) to reduce network activity.
  • NMDA currents were evoked by applying NMDA directly to the recording chamber during continuous perfusion.
    • To test the selectivity of NMDA to NMDA receptors the experiment was repeated within the same neuron in the presence of the NMDA receptor antagonist D-AP5 (100 μM). Under these conditions NMDA failed to induce a depolarising current.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Chemical Data

Chemical name N-Methyl-D-aspartic acid
Molecular Weight 147.13
Chemical structure NMDA  [6384-92-5] Chemical Structure
Molecular Formula C5H9NO4
CAS Number 6384-92-5
PubChem identifier 22880
SMILES CN[C@H](CC(=O)O)C(=O)O
InChi InChI=1S/C5H9NO4/c1-6-3(5(9)10)2-4(7)8/h3,6H,2H2,1H3,(H,7,8)(H,9,10)/t3-/m1/s1
InChiKey HOKKHZGPKSLGJE-GSVOUGTGSA-N
MDL number MFCD00004226

References for NMDA

References are publications that support the biological activity of the product
  • Regulation of N-methyl-D-aspartic acid (NMDA) receptors by metabotropic glutamate receptor 7.

    Gu Z et al (2012) J Biol Chem 287(13) : 10265-75.
  • N-methyl-D-aspartic acid (NMDA) in the nervous system of the amphioxus Branchiostoma lanceolatum.

    D'Aniello S et al (2007) BMC Neurosci 8 : 109
  • Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine tissues and their role in the modulation of luteinizing hormone and growth hormone release.

    D'Aniello A et al (2000) FASEB J 14(5) : 699-714.
Publications
These publications cite the use of NMDA purchased from Hello Bio:
  • Long-term synaptic depression triggers local biogenesis of autophagic vesicles in dendrites and requires autophagic degradation

    Kallergi et al. (2020) bioRxiv preprint : 1-46

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