Figure 1. Cyclothiazide enhancement of evoked EPSCs in mouse cortical neuron
The AMPAR positive allosteric modulator Cyclothiazide increases the open time of the AMPAR by inhibiting AMPAR desensitization. It is often used at a concentration of 100 µM. Cyclothiazide from Hello Bio potentiates AMPAR mediated evoked EPSCs in cortical neurons at 50 µM and above. For assay protocol, see #Protocol 1 in Application Notes below.
Cyclothiazide: Scientist Approved
Cyclothiazide product vial image | Hello Bio
Figure 1. Cyclothiazide enhancement of evoked EPSCs in mouse cortical neuron
The AMPAR positive allosteric modulator Cyclothiazide increases the open time of the AMPAR by inhibiting AMPAR desensitization. It is often used at a concentration of 100 µM. Cyclothiazide from Hello Bio potentiates AMPAR mediated evoked EPSCs in cortical neurons at 50 µM and above. For assay protocol, see #Protocol 1 in Application Notes below.
Cyclothiazide: Scientist Approved
Cyclothiazide product vial image | Hello Bio
Biological Data
Biological description
Cyclothiazide (CTZ) is an AMPA receptor positive allosteric modulator (PAM) which produces a fast inhibition of AMPAR desensitization and a slow potentiation of the AMPA current.
Cyclothiazide also inhibits GABAA mediated currents and shows diuretic and convulsive actions.
Cyclothiazide is active in vivo.
Application notes
The AMPAR positive allosteric modulator Cyclothiazide increases the open time of the AMPAR by inhibiting AMPAR desensitization. It is often used at a concentration of 100 μM. Cyclothiazide from Hello Bio potentiates AMPAR mediated evoked EPSCs in cortical neurons at 50 μM and above (see Fig 1 above)
#Protocol 1: Evoked and spontaneous evoked excitatory post synaptic currents (EPSCs)
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
Neurons were held at -70 mV (the reversal potential of GABA currents).
EPSCs were then continuously stimulated and recorded in response to 5 min applications of 50 μM and then 100 μM Cyclothiazide.
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Soluble in DMSO (100mM) or ethanol (25mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
These publications cite the use of Cyclothiazide purchased from Hello Bio:
Calcium rises induced by AMPA and nicotine receptors in the ventral tegmental area show differences in mouse brain slices prenatally exposed to nicotine.
Ipsen et al (2018) Dev Neurobiol doi:10. : 1002/dneu.22607.
![Cyclothiazide [2259-96-3] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0221.png "Cyclothiazide [2259-96-3]")
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