CHIR 99021 trihydrochloride

Technical documents: SDS CoA Datasheet

Product overview

Name CHIR 99021 trihydrochloride
Purity >98%
Description Potent, selective GSK3 inhibitor and Wnt signaling activator. Commonly used in organoid production and involved in reprogramming MEFs to IPSCs and fibroblasts to mature neurons. Water soluble.
Write Your Own Review
You're reviewing:CHIR 99021 trihydrochloride
Rate this item:

Biological Data

Biological description

Potent, selective and ATP-competitive GSK3 β inhibitor (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively).

Water soluble hydrochloride salt of CHIR 99021.

Wnt signaling activator which is commonly used with PD 032501 as part of the 2i condition.

Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over 20+ other enzymes and receptors.

Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.

Commonly used in organoid production and also involved in reprogramming of fibroblasts to mature neurons.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in water (25mM) and in DMSO (50mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



More Info


More Info

Chemical Data

Purity >98%
Chemical name 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethy l]amino]-3-pyridinecarbonitrile trihydrochloride
Molecular Weight 574.72
Chemical structure CHIR 99021 trihydrochloride [1782235-14-6] Chemical Structure
Molecular Formula C22H18Cl2N8.3HCl
CAS Number 1782235-14-6
PubChem identifier 78243722
Source Synthetic
InChi InChI=1S/C22H18Cl2N8.3ClH/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19;;;/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32);3*1H
MDL number MFCD11846251

References for CHIR 99021 trihydrochloride

References are publications that support the biological activity of the product
  • The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.

    Li C et al (2013) PLoS One 8(12) : e84659.
  • Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.

    Ye S et al (2012) PLoS One 7(4) : e35892.
  • Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.

    Li W et al (2009) Stem cells 27(12) : 2992-3000

3 Item(s)

These publications cite the use of CHIR 99021 trihydrochloride purchased from Hello Bio:
  • In vitro modelling of human microglial alterations associated with Alzheimer's disease and polygenic risk

    Salis et al (2022) Cardiff University : Thesis

1 Item