Potent, selective GSK3 inhibitor and Wnt signaling activator. Commonly used in organoid production and involved in reprogramming MEFs to IPSCs and fibroblasts to mature neurons. Water soluble.
Wnt signaling activator which is commonly used with PD 032501 as part of the 2i condition.
Exhibits no cross reactivity against CDKs and exhibits >500-fold selectivity for GSK3 over other protein kinases and >800-fold selectivity over 20+ other enzymes and receptors.
Promotes self-renewal of embryonic stem cells and enables mouse embryonic fibroblast (MEF) reprogramming into iPSCs.
Commonly used in organoid production and also involved in reprogramming of fibroblasts to mature neurons.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in water (25mM) and in DMSO (50mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References are publications that support the biological activity of the product
The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways.
Potent, selective GSK3 inhibitor and Wnt signaling activator. Commonly used in organoid production and involved in reprogramming MEFs to IPSCs and fibroblasts to mature neurons. Water soluble.
Y-27632 is a selective ROCK inhibitor. Frequently used 3D growth matrix component and for production of organoids. Also used when inducing neurons from fibroblasts. Manufactured to a high degree of optical purity.
![CHIR 99021 trihydrochloride [1782235-14-6] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb1262.png "CHIR 99021 trihydrochloride [1782235-14-6]")
Understanding purity and quality - a guide for life scientists
