The GABAA receptor agonist muscimol is used at concentrations of 1-50 µM. Muscimol from Hello Bio used at 10 µM led to a large hyperpolarising whole-cell current in hippocampal CA1 neurons (see Fig 1 above). Action of muscimol was fully blocked by the GABAA receptor antagonist bicuculline (100 µM).
#Protocol 1: Assay used for muscimol
Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
Neurons were held at 0 mV and GABAA receptor currents were evoked via applying muscimol directly to the recording chamber during continuous perfusion.
To test muscimol’s selectivity to GABAA receptors the experiment was repeated within the same neuron in the presence of the GABAA receptor antagonist bicuculline (100 µM).
Under these conditions muscimol failed to induce a hyperpolarising current.
Solubility & Handling
Soluble in water (100mM)
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