Muscimol

(HB0887)
Technical documents: SDS CoA Datasheet

Product overview

Name Muscimol
Description Potent, selective, competitive GABAA receptor agonist
Biological description

Potent, selective and competitive GABAA receptor agonist and a potent partial GABAA-ρ (GABAC) receptor agonist.


Muscimol is a GABA analog with comparable potency to GABA and is thought to act at the orthosteric site at GABAA receptors in varying active conformations.


Also acts as a weak inhibitor of GABA uptake but is not a substrate for GABA transaminase.


Application of muscimol evokes GABAAR currents and its actions are antagonized by the GABAAR antagonist bicuculline (bicuculline methochloride, methiodide and methobromide also available).

Muscimol enhances inhibitory neurotransmission and suppresses spontaneous activity. It is commonly used in reversible brain inactivation studies.


Active in vivo and blood brain barrier permeable.

Shows psychoactive, memory impairing effects and anticonvulsant actions at high doses.

Purity >99%
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Biological Data

Application notes

The GABAA receptor agonist muscimol is used at concentrations of 1-50 µM. Muscimol from Hello Bio used at 10 µM led to a large hyperpolarising whole-cell current in hippocampal CA1 neurons (see Fig 1 above). Action of muscimol was fully blocked by the GABAA receptor antagonist bicuculline (100 µM).


#Protocol 1: Assay used for muscimol

  • Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
  • Neurons were held at 0 mV and GABAA receptor currents were evoked via applying muscimol directly to the recording chamber during continuous perfusion.
  • To test muscimol’s selectivity to GABAA receptors the experiment was repeated within the same neuron in the presence of the GABAA receptor antagonist bicuculline (100 µM).
  • Under these conditions muscimol failed to induce a hyperpolarising current.

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (100mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name 5-Aminomethyl-3-hydroxyisoxazole
Molecular Weight 114.1
Chemical structure Product image
Molecular Formula C4H6N2O2
CAS Number 2763-96-4
PubChem identifier 4266
SMILES C1=C(ONC1=O)CN
Source Synthetic
InChi InChI=1S/C4H6N2O2/c5-2-3-1-4(7)6-8-3/h1H,2,5H2,(H,6,7)
InChiKey ZJQHPWUVQPJPQT-UHFFFAOYSA-N
MDL number MFCD00057894
Appearance White solid

References for Muscimol

References are publications that support the biological activity of the product
  • Anticonvulsant and behavioral effects of muscimol in immature rats.

    Mareš P et al (2014) Brain Res 1582 : 227-36.
  • Muscimol as an Ionotropic GABA Receptor Agonist.

    Johnston GA (2014) Neurochem Res 39(10) : 1942-7.
  • Hippocampal infusions of pyruvate reverse the memory-impairing effects of septal muscimol infusions.

    Krebs DL et al (2005) Eur J Pharmacol 520(1-3) : 91-9.
Publications
These publications cite the use of Muscimol purchased from Hello Bio:
  • mGluR-LTD at Excitatory and Inhibitory Synapses in the Lateral Habenula Tunes Neuronal Output.

    Valentinova and Mameli (2016) Cell Rep 16(9) : 2298-307
    PubMedID: 27545888
  • Non-invasive molecularly-specific millimeter-resolution manipulation of brain circuits by ultrasound-mediated aggregation and uncaging of drug carriers

    Ozdas et al (2020) Nat Commun. 11(1) : 4929.
    PubMedID: 33004789

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