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Figure 1. Kynurenic acid sodium salt inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10 mM. Kynurenic acid sodium salt from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10 mM. For assay protocol, see #Protocol 1 in Application Notes below.
Inhibition of AMPA and NMDA mediated responses by kynurenic acid
Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10 mM. Kynurenic acid sodium salt from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10 mM. For assay protocol, see #Protocol 1 in Application Notes below.
Figure 2. Percentage inhibiton of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
See Alt et al., 2004 for methodology and protocol
Kynurenic acid sodium salt: Scientist Approved
Kynurenic acid sodium salt product vial image | Hello Bio
Figure 1. Kynurenic acid sodium salt inhibition of evoked NMDAR mediated EPSCs in mouse cortical neuron
Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10 mM. Kynurenic acid sodium salt from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10 mM. For assay protocol, see #Protocol 1 in Application Notes below.
Inhibition of AMPA and NMDA mediated responses by kynurenic acid
Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10 mM. Kynurenic acid sodium salt from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10 mM. For assay protocol, see #Protocol 1 in Application Notes below.
Figure 2. Percentage inhibiton of glutamate (30 µM) stimulated increase of Ca2+ fluorescence in HEK293 cells expressing GluK2
See Alt et al., 2004 for methodology and protocol
Kynurenic acid sodium salt: Scientist Approved
Kynurenic acid sodium salt product vial image | Hello Bio
Biological Data
Biological description
Endogenous, non-selective ionotropic glutamate receptor antagonist which acts as a non-competitive glycine site NMDAR antagonist. Also an α7 nicotinic receptor antagonist and GPR35 ligand. Sodium salt. Blocks kainic acid neurotoxicity and displays neuroprotective, antiproliferative and antimigrative properties.
Application notes
Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10mM. Kynurenic acid from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10mM (see Fig 1 above).
#Protocol 1: Evoked NMDA receptor currents
Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg2+ block.
NMDA currents were continually stimulated and recorded in response to continual bath applications of NMDAR antagonists until NMDA currents were completely abolished. If the concentration was not effective an addition experiment was conducted using an alternative concentration.
All NMDAR recordings were made in the presence of GABAA-R and AMPAR antagonists.
Solubility & Handling
Storage instructions
Room temperature (desiccate)
Solubility overview
Soluble in water (100mM) or DMSO (50mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![Kynurenic acid sodium salt [2439-02-3] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb0363.png "Kynurenic acid sodium salt [2439-02-3]")
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