Kynurenic acid sodium salt

Kynurenic acid is commonly used as an AMPA/NMDA receptor antagonist. Kynurenic acid is commonly used at concentration of 10mM. Kynurenic acid from Hello Bio rapidly inhibits both NMDA and AMPA mediated spontaneous and evoked EPSCs at concentrations of 10mM (see Fig 1 above).

#Protocol 1: Evoked NMDA receptor currents

• Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
• NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
• Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg²⁺ block.
• NMDA currents were continually stimulated and recorded in response to continual bath applications of NMDAR antagonists until NMDA currents were completely abolished. If the concentration was not effective an addition experiment was conducted using an alternative concentration.
• All NMDAR recordings were made in the presence of GABA_A-R and AMPAR antagonists.